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Biointerphases

Journal for Biophysical Chemistry

Fig. 6 | Biointerphases

Fig. 6

From: Micrometer and Nanometer Scale Photopatterning of Proteins on Glass Surfaces by Photo-degradation of Films Formed from Oligo(Ethylene Glycol) Terminated Silanes

Fig. 6

Images of nanopatterns formed by interferometric exposure of monolayers of OEG-silane on glass. a, b FFM images of a sample exposed for 6 min at 50 mW cm−2. c, d Tapping mode AFM topographical images of similar samples to those in a and b, following immersion in a solution of streptavidin in PBS. e Tapping mode phase image of a sample exposed at the same power for 10 min following adsorption of streptavidin. Image size: 10 × 10 μm2, z-contrast range 0–50° dark to bright. f, g Confocal fluorescence microscopy images of two regions of a sample formed by interferometric exposure of an OEG-silane film with 2θ = 20°, NTA functionalization, Ni2+ complexation, and incubation in a solution of His-tagged GFP

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