Figure 5
From: Fluorescence modulation sensing of positively and negatively charged proteins on lipid bilayers
Titration curves for two buffer conditions with supported POPC bilayers containing 0.5 mol% ortho -rhodamine B and 5 mol% biotin-PE. The y-axis values were obtained from the intensity profiles at each pH divided by the maximum fluorescence intensity for the titration at low pH. The solid circles represent individual fluorescence measurements and the solid lines are sigmoidal fits to the data.