Fig. 8

Culture of mOBs on melt electrospun PCL fibers taken from a tube with a winding angle of 30°. mOBs initially showed good attachment and then proliferated over a culture period of 4 weeks. a After 4 weeks of culture a 2 mm biopsy punch was used to harvest the specimen shown in b–d. SEM showed that the mOBs formed a mineralized ECM not only on the scaffold fibers but also inside and across the pore architecture (b and c). d CLSM revealed by using DAPI/Phalloidin staining alignment of mOBs along the fiber axis