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Journal for Biophysical Chemistry

Fig. 2 | Biointerphases

Fig. 2

From: Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Fig. 2

Methodology for fluorescence quantification of CLSM images. Regions of interest (ROIs; green outlines) were hand-drawn on overlapped transmission (left) and red channel images (right) for each treatment. ROIs were drawn around the cell membrane, nucleus and the background of each image. Final fluorescence intensities were calculated from the red channel only (right). The final cytoplasmic fluorescence intensities were calculated as described in Sect. 2

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