Fig. 2From: Dynamic Cellular Uptake of Mixed-Monolayer Protected NanoparticlesMethodology for fluorescence quantification of CLSM images. Regions of interest (ROIs; green outlines) were hand-drawn on overlapped transmission (left) and red channel images (right) for each treatment. ROIs were drawn around the cell membrane, nucleus and the background of each image. Final fluorescence intensities were calculated from the red channel only (right). The final cytoplasmic fluorescence intensities were calculated as described in Sect. 2Back to article page