Fig. 6
From: Model Membrane Platforms for Biomedicine: Case Study on Antiviral Drug Development
Mechanism of AH peptide interaction with lipid vesicles. Combined QCM-D and reflectometry analysis for AH peptide-mediated structural transformation from intact vesicle layer to planar bilayer on a gold surface. a QCM-D resonance frequency data of structural transformation process. Multiple overtones, z, up to the 13th, were recorded. b QCM-D energy dissipation data of structural transformation process. The data demonstrate the changes in viscoelasticity of the film during the vesicle-to-bilayer transformation. c Simultaneously, the reflectometric response was also obtained. d Acoustic mass (red long-dotted line) and optical mass signatures (black line) of the structural transformation can elucidate the mass contributed by bound solvent (blue dot). Parameters used for calculating the optical mass and solvent mass were ρsolvent = 1.0 g m−3, r = 0.286 cm3 g, ρ = 1.02 g cm−3, v = 0.98 cm3 g−1, and n0 = 1.334. e Magnified view of the graph in part d. Note that there is a break on the y-axis from 1,290 to 4,500 ng/cm−2, the lower and upper scales differ by a factor of 15, and the data are only plotted from 54 to 70 min. f Hydration signatures of vesicle rupture on gold (Au) and silicon oxide (SiOx). The hydration signature helps to describe the mechanism of structural transformation processes. Figure is adapted and modified from Ref. [43]