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Biointerphases

Journal for Biophysical Chemistry

Fig. 2 | Biointerphases

Fig. 2

From: Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

Fig. 2

Differentially treated polymer films alter surface presentation of L1-Fc. a Schematic representation of how L1-Fc might randomly orient when coated onto a layer of PDL. b PA presentation will result in four L1-Fc molecules per PA molecule [68], enabling the multimeric, oriented presentation of L1-Fc. c AFM images (1 × 1 μm2, two and three dimensional) and cross-sectional profiles of the protein complexes, which correspond to the white lines drawn on the images, of thin polymer films coated with L1-Fc/PDL, L1-Fc/PA/PDL, or L1-Fc/PA. Thin films in which L1-Fc is presented from PA revealed increased L1-Fc adsorption as shown by the increase in protein complexes in the 2-D and 3-D images. PA presented L1-Fc resulted in cross-sectional profiles with wider peaks compared to L1-Fc adsorbed on PDL. A summary of the thickness of the thin films and the width of the protein complexes is shown in the tables to the far right. PDL poly-d-lysine, PA protein A (* denotes p < 0.05 compared to both PDL and PA/PDL controls, § denotes p < 0.05 compared to PA control, # denotes p < 0.05 compared to the L1-Fc/PDL condition)

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