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Biointerphases

Journal for Biophysical Chemistry

Fig. 3 | Biointerphases

Fig. 3

From: Oriented, Multimeric Biointerfaces of the L1 Cell Adhesion Molecule: An Approach to Enhance Neuronal and Neural Stem Cell Functions on 2-D and 3-D Polymer Substrates

Fig. 3

Protein A presentation of L1-Fc enhances neurite outgrowth in spinal cord and cerebellar neurons. a Spinal cord neurons (SCNs) were plated on L1-Fc adsorbed onto PDL (L1-Fc/PDL), a combination of PDL and PA (L1-Fc/PA/PDL), and PA alone (L1-Fc/PA), as well as control surfaces that did not contain L1-Fc. SCNs cultured for 24 h on L1-Fc/PA/PDL or L1-Fc/PA extended longer neurites compared to those on L1-Fc/PDL treated films. b Quantification of the average total neurite outgrowth indicated that on 10 or 50 μg/ml of L1-Fc, there was no significant difference in outgrowth between the two conditions in which L1-Fc was presented from protein A (with or without PDL). c Quantification of the average length of the longest neurite per cell. d Morphology of CNs that were seeded onto the same substrates listed above, where 10 μg/mL of L1-Fc was used. e Substrates in which L1-Fc was presented using PA resulted in enhanced neurite outgrowth of CNs compared to neurons cultured on L1-Fc presented from PDL. Scale bar 75 μm. PDL poly-d-lysine, PA protein A (* denotes p < 0.05, ** denotes p < 0.01)

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