Fig. 1
From: Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor
A schematic presentation of RGD and EGF surface functionalization. Plasma -etched glass slides (a) are incubated with triethoxysilane–PEG–biotin in toluene, resulting in the formation of biotinylated glass slides (b). The biotinylated glass slides are further incubated with preformed NeutrAvidin (NT)–biotin–EGF/RGD complexes at various complex concentrations, resulting in the formation of glass cover slips biofunctionalized with RGD (c) or with a mixture of RGD and EGF at different densities and ratios (d)