Fig. 2
From: Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor
A431 cell spreading on RGD-biofunctionalized surfaces at varying densities. The fluorescent images in the top panel are of actin-labeled A431 cells; the numbers on each image indicate the RGD density in pmol/cm2 (a), scale bar 15 μm. The graphs in the bottom panel display the 90th percentiles of cell aspect ratio (b) and cell area (c). All results are expressed as mean ± confidence intervals (CI). Aspect ratio and area of cells plated on 6 and 12 pmol/cm2 RGD surfaces that significantly differed from 46 pmol/cm2 RGD surfaces at the P-value levels P < 0.05 and P < 0.01 are denoted by one and two asterisks, respectively