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Journal for Biophysical Chemistry

Fig. 3 | Biointerphases

Fig. 3

From: Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor

Fig. 3

Focal adhesion growth and actin filament reorganization in A431 cells exposed to soluble EGF. Cells were plated on surfaces modified with 184 pmol/cm2 RGD. Five hours after cell plating, EGF was added to the medium for 30 min. A separate cell sample was incubated for 5.5 h without EGF, as a control. After 5.5 h, both samples were fixed and stained for zyxin at focal adhesions and for actin filaments. The graphs in the top panel indicate the 90th percentile of the focal adhesion elongation (a), the average number of focal adhesions per cell (b), and area (c) as a function of EGF concentration. All results are expressed as mean ± confidence intervals (CI). Parameters of FAs in cells that were exposed to soluble EGF and significantly differed from the corresponding parameters of FAs in control cell, not exposed to EGF, at the P-value levels P < 0.05 and P < 0.01 are denoted by one and two asterisks, respectively. Fluorescent images of actin- and zyxin-labeled cells exposed to 1 and 10 ng/ml, in comparison to control (no EGF) are presented in the bottom panel (d). Scale bar 15 μm

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