Skip to main content

Journal for Biophysical Chemistry

Fig. 4 | Biointerphases

Fig. 4

From: Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor

Fig. 4

The effect of RGD density on cell adhesion in response to soluble EGF. Average number of focal adhesions per cell as a function of EGF concentration on surfaces with different RGD densities (a), the numbers at the top right of the graphs are the RGD density in pmol/cm2. Low bars with dashed borders represent a scenario where cells detached from the surface. The insert in a, at the top left represents the same data as that seen in the main graph, where the average number of focal adhesions per cell are plotted as a function of the surface RGD density. The numbers at the top of each graph represent the applied EGF concentrations in ng/ml. All results are expressed as mean ± confidence intervals (CI). Number of FAs in cells plated on 46, 92 and 184 pmol/cm2 RGD surfaces that were significantly higher than the number of FAs in cells plated on both 6 and 12 pmol/cm2 RGD surfaces at the P-value levels P < 0.05 and P < 0.002 are denoted by one and two asterisks, respectively. The images in the bottom panel are of A431 cells, plated on surfaces modified with RGD at different densities, following stimulation with 1 ng/ml EGF. The number on each image indicates the RGD density in pmol/cm2. The experimental procedure was the same as that described in the legend to Fig. 3. Scale bar 15 μm

Back to article page