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Journal for Biophysical Chemistry

Fig. 4 | Biointerphases

Fig. 4

From: Platelet Activation Profiles on TiO2: Effect of Ca2+ Binding to the Surface

Fig. 4

Summary of the platelet activation profiles under different conditions employed in this study. Shown in this figure is the background-subtracted fluorescence intensity, [(S − B)/B], where S is signal and B is background, in the red channel, corresponding to the expression of the activation markers CD62P or CD63 under different conditions used in this study. For each condition, the data was collected from at least three experiments using the blood of three different donors. The trends confirm those apparent in the individual images shown in Figs. 2 and 3. Note the change in scale: while in a–c, the fluorescence intensity is plotted on a scale from 0 to 100 %, in d and e it is plotted on the scale from 0 to 1 %, because the average intensity is so low under these conditions. a Expression of CD62P in platelets adhering on TiO2 and on glass in the absence of Ca2+. Significant levels of CD62P expression are seen on glass. On the other hand the expression is not detectable on TiO2 after 10 min. After 3 h, the spread platelets do not express CD62P on TiO2, while the non-spread platelets do express some. b Expression of CD62P in platelets adhering on TiO2 and on glass in the presence of Ca2+. CD62P is expressed on platelets adhering on both surfaces after 10 min and after 3 h. c Platelets express CD63 in the absence of Ca2+ on both surfaces. d, e Chelation of intracellular Ca2+ by BAPTA-AM affects the expression of both activation markers. Note the change in scale compared to a–c

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