Fig. 1

Surface coating modulates the adhesion of neurons. aBar plot showing the number of neurons adhered onto substrates with different coatings at 4 h after plating. The concentrations of the coating agents were 50 µg/mL except for MG, which was diluted to 1:100 v/v. MG: n = 6, LN: n = 4, FN: n = 6, PEI: n = 10, PDL: n = 5, PLL: n = 5. b Pair-wise comparisons between neuronal adhesion on different types of surfaces show that the number of neurons adhered onto different substrates are significantly different (Kruskal–Wallis ANOVA followed by Tukey–Kramer’s post hoc multiple comparisons tests against the same dataset in a). Red error bars denote no significant difference, and this applies to FN against LN, and to any combination between PEI, PDL and PLL. Yellow error bars denote difference that are significant (0.01 < P < 0.5) depending on the standard chosen, and this applies to FN or LN against MG. Blue error bars denote significant difference (P < 0.001), and this applies to any combination between PEI, PDL or PLL against FN, LN or MG. Yellow shaded area highlights the significance zone. c The number of neurons adhered depends on the concentration of the coating agents (Students’ t test, P < 0.001 for each pair, numbers of samples at 25 µg/mL, LN: n = 6, FN: n = 6, PEI: n = 10, PDL: n = 6, PLL: n = 5, numbers of samples at 50 µg/mL, same as those in a); Error bars SEM