Fig. 3

Comparison of neuronal morphology on different surfaces at 12 h after plating. a–cBar plot showing the somata area, neurite number and average neurite length of neurons on various surfaces (MG: n = 5, 5 data points; PEI 50 µg/mL: n = 5, 30 data points; PDL 50 µg/mL: n = 3, 76 data points; LN 50 µg/mL: n = 3, 32 data points; PLL 50 µg/mL: n = 3, 45 data points). d–f Multiple comparisons between different between different surface coating conditions corresponding to a–c. Somata areas and neurite numbers of neurons are significantly on various substrates (P < 0.01 for blue error bars, P > 0.05 for red error bars) while neurite lengths are not significantly different (Kruskal–Wallis ANOVA followed by Bonferroni’s post hoc multiple comparisons tests). g–i Somata areas, neurite numbers and neurite lengths are not significantly different between surface coating agents at 25 and 50 µg/mL. (PEI 25 µg/mL: n = 5, 50 data points; PDL 25 µg/mL: n = 14, 60 data points; LN 25 µg/mL: n = 5, 15 data points; PLL 25 µg/mL: n = 9, 55 data points. Student’s t test). Error bars SEM