Fig. 4

Comparison of the morphology of neurons between PLL and MG at 24 h after plating. a–b Immunofluorescence photomicrographs of neurons on MG (a) and PDL (b) with neuron specific marker Tuj1, cell nuclei counterstained with Hoechst. Neurons on PDL surfaces develop more neurites, have shorter neurites, and the longest neurites are shorter compared to neurons on MG. See text for details. c–f Somata areas, neurite numbers, neurite lengths are the length of the longest neurite for each neuron are significantly different on MG and PDL (c MG, n = 5, PLL, n = 15; d MG, n = 17, PLL, n = 17; e MG, n = 11, PLL, n = 16; f MG, n = 5, PLL, n = 5. Mann–Whitney U test, **P < 0.01). Scale bars 200 µm