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Journal for Biophysical Chemistry

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Table 1 Reaction time, grafting yield, and surface composition of the pristine PCL and surface-functionalized PCL surfaces

From: Immobilization of Gelatin onto Poly(Glycidyl Methacrylate)-Grafted Polycaprolactone Substrates for Improved Cell–Material Interactions

Sample Reaction time (h) GY (μg/cm2) (mean ± SD) [Br]/[C]j [N]/[C]j
Pristine PCLa
PCL-NH b2 1 0.043
PCL-GAc 12
PCL-gelatind 24 0.42 ± 0.11 0.097
PCL-Bre 12 3.17 × 10−2
PCL-g-P(GMA)0f 0.5 2.72 ± 0.68 2.34 × 10−2
PCL-g-P(GMA)1g 1 6.31 ± 1.32 9.29 × 10−3
PCL-g-P(GMA)2h 3 14.76 ± 2.63 4.72 × 10−3
PCL-g-P(GMA)0-c-gelatini 24 0.93 ± 0.25  
PCL-g-P(GMA)1-c-gelatini 24 2.63 ± 0.52 0.169
PCL-g-P(GMA)2-c-gelatini 24 3.79 ± 0.73 0.202
  1. SD standard deviation
  2. aPristine PCL refers to the cleaned PCL film after rigorous washing with alcohol and deionized water
  3. bPCL-NH2 was obtained after 1 h of aminolysis in a 10 % (w/w) 1,6-hexanediamine/isopropanol mixture at 40 °C
  4. cPCL-GA was obtained after the PCL-NH2 surface immersed in a 2.5 wt% PBS solution of glutaradehyde (GA) at room temperature for 12 h
  5. dPCL-gelatin refers to the PCL-GA surface conjugated with gelatin after incubated in the PBS (pH 7.4) solution containing the gelatin at a concentration of 3 mg/ml at room temperature for 24 h
  6. ePCL-Br was obtained after the PCL-NH2 surface reacted with 2-bromoisobutyryl bromide (BIBB) in dried hexane containing 1:1 (molar ratio) BIBB and triehtylamine (TEA)
  7. f,gReaction conditions: [GMA]:[CuBr]:[CuBr2]:[bpy] = 100:1:0.2:2 in methanol/water solution (1:1, v:v) at room temperature for 1 and 3 h to produce the PCL-g-P(GMA)1 and PCL-g-P(GMA)2 surfaces, respectively
  8. hReaction conditions: the PCL-g-P(GMA)1 and PCL-g-P(GMA)2 surfaces incubated in the PBS (pH 7.4) solution containing the gelatin at a concentration of 3 mg/ml at room temperature for 24 h
  9. iGY denotes grafting yield, and is defined as GY = (W a  − W b )/A, where W a and W b corresponds to the weight of the dry films before and after grafting, respectively, and A is the film area (about 3.2 cm2)
  10. jDetermined from their corresponding sensitivity factor-corrected element core-level spectral area ratios
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