Journal for Biophysical Chemistry
Table 1 Compilation of the protein elution doses (%), their corresponding elution times (expressed as the time it starts eluting till it finishes) and the protein elution span (Δt) for the two columns i.e., mHA-C and mHA-F
From: Assessment of Protein Entrapment in Hydroxyapatite Scaffolds by Size Exclusion Chromatography
| Â | C, elution (%) | F, elution (%) | ||
|---|---|---|---|---|
| Â | 1st peak | 2nd peak | 1st peak | 2nd peak |
γ-G | 67.0 ± 2 | 31.0 ± 2.7 | 79.0 ± 6.7 | 21.0 ± 6.7 |
BSA | 55.5 ± 1.3 | 44.2 ± 1.1 | 70.5 ± 1.5 | 29.0 ± 1.5 |
β-LG | 78.6 ± 1.5 | – | 71.6 ± 0.2 | – |
| Â | C, elution t (min) | F, elution t (min) | ||
|---|---|---|---|---|
| Â | 1st peak | 2nd peak | 1st peak | 2nd peak |
γ-G | 1.2–4.7 | 3.8–13 | 1.2–4.7 | 5.6–20 |
BSA | 1.2–4.5 | 7–18 | 1.2–4.5 | 8.3–20 |
β-LG | 1.2–9.2 | 11.7–18.7 | 1.2–9.2 | 14.3–19.9 |
|  | C, elution ∆t (min) | F, elution ∆t (min) | ||
|---|---|---|---|---|
| Â | 1st peak | 2nd peak | 1st peak | 2nd peak |
γ-G | 3.5 | 9.2 | 3.5 | 14.4 |
BSA | 3.3 | 11 | 3.3 | 11.7 |
β-LG | 8 | 7 | 8 | 5.6 |