Correlations Between the Biochemistry and Mechanical States of a Sea-Urchin Ligament: A Mutable Collagenous Structure

Biointerphases

Journal for Biophysical Chemistry

Table 2 Proteins identified in ECM of CLD from the sea-urchin Paracentrotus lividus identified by MALDI–TOF/TOF–MS of in-gel tryptic digests of the bands indicated in Fig. 4a

Gel band	Protein name	Species	Accession number^a	Database	Protein score^b	Peptide score^b	Number of distinct peptides^c
1	Predicted similar to cytoskeletal actin	Strongylocentrotus purpuratus	gi\|72007954	Uniprot-SwissProt/S.p.	791	662	16
2	Predicted similar to cytoskeletal actin	Strongylocentrotus purpuratus	gi\|72007954	Uniprot-SwissProt/S.p.	749	631	15
3	Tubulin alpha-1 chain	Paracentrotus lividus	P18258	Uniprot-SwissProt/S.p.	569	496	11
4	Predicted similar to myosin heavy chain	Strongylocentrotus purpuratus	gi\|115692122	Uniprot-SwissProt/S.p.	661	580	23
5	Predicted similar to major yolk protein precursor	Strongylocentrotus purpuratus	gi\|115924727	Uniprot-SwissProt/S.p.	137	118	8
	Toposome	Paracentrotus lividus	Q6WQT5	UniRef100	265	151	17
6	Major yolk protein	Strongylocentrotus purpuratus	gi\|47551123	Uniprot-SwissProt/S.p.	226	212	9
	Toposome	Paracentrotus lividus	Q6WQT5	UniRef100	873	673	32
7	Predicted similar to myosin heavy chain	Strongylocentrotus purpuratus	gi\|115692122	Uniprot-SwissProt/S.p	900	766	31

Peptide mass (MS) and fragmentation (MS/MS) data were used to search against the UniProt/Swiss-Prot database joined together with the purple sea urchin Strongylocentrotus purpuratus genome predicted protein database and UniRef100. A confident index of 100 % was obtained in all cases
^aAccession numbers of the identified protein in UniProt-SwissProt/S.p. or Uniref100 databases
^bMowse scoring algorithm corresponding to a confidence index for which the protein/peptide match was not random
^cPeptide whose sequence differs in at least 1 amino acid residue