RETRACTED ARTICLE: Plasma Protein Adsorption to Zwitterionic Poly (Carboxybetaine Methacrylate) Modified Surfaces: Chain Chemistry and End-Group Effects on Protein Adsorption Kinetics, Adsorbed Amounts and Immunoblots

Biointerphases

Journal for Biophysical Chemistry

Table 3 Relative intensities for immunoblots of plasma proteins eluted from the PCBMA systems and amount of plasma proteins eluted from PCBMA-functionalized silica wafers determined using the Bio-Rad DC microplate protein assay

Plasma protein	Fragment size (kDa)	System
Plasma protein	Fragment size (kDa)	PCBMA-1 (Phospho)	PCBMA-3 (Phospho)	PCBMA-5 (Phospho)	PCBMA-1 (TEMPO)		PCBMA-3 (TEMPO)	PCBMA-5 (TEMPO)
Albumin	66	*****	*****−	****+	*****−	*		****
Alpha1 Antitrypsin	47	****	*	*	*	*		*
Transferrin	77	*****−	*	*	*	*		*
Total eluted protein per surface area (μg/cm²)^a		5.4	3.8	2.9	7.4	6.5		6.3

One star indicates zero band intensity. Five stars indicate a high intensity band. +and – indicate a slightly greater or lesser band intensities respectively
^aData represent average of n = 3, all have SD of <10 % of the average value. Note that these values are on the lower range of the detection limit of the total protein assay employed, and are shown to illustrate the trend in adsorption