Journal for Biophysical Chemistry
Table 1 Primary antibodies used for immunoblotting of plasma proteins adsorbed to bioactive glass as well as plasma protein physical characteristics
Antibody | Plasma protein MW (kDa) | Plasma protein pI | Host | Source |
|---|---|---|---|---|
Kininogen (light chain) | 50 | 6.6 | Mouse | US Biological, Swampscott, MA, USA |
Kininogen (heavy chain) | 88–120 | 6.8 | Mouse | US Biological, Swampscott, MA, USA |
Factor I | 88 | 7.6 | Mouse | Cedarlane Laboratories, Hornby, ON, CAN |
Fibrinogen | 340 | 6.6 | Rabbit | Calbiochem, Gibbstown, NJ, USA |
Fibronectin | 440 | 5.7 | Rabbit | Cedarlane Laboratories, Hornby, ON, CAN |
Hemoglobin | 68 | 8.0 | Rabbit | Sigma-Aldrich, St. Louis, MO, USA |
α1-Antitrypsin | 47 | 5.6 | Sheep | Cedarlane Laboratories, Hornby, ON, CAN |
Thrombin | 36 | 5.4 | Sheep | Cedarlane Laboratories, ON, CAN |
Prothrombin | 72 | 5.9 | Sheep | Cedarlane Laboratories, ON, CAN |
Protein C | 62 | 6.3 | Sheep | Cedarlane Laboratories, ON, CAN |
Vitronectin | 75 | 5.8 | Sheep | Cedarlane Laboratories, ON, CAN |
Protein S | 69 | 5.7 | Sheep | Cedarlane Laboratories, ON, CAN |
Prekallikrein | 85 | 8.2 | Sheep | Cedarlane Laboratories, ON, CAN |
Antithrombin | 53 | 6.7 | Sheep | Cedarlane Laboratories, ON, CAN |
IgG | 174 | 8.4 | Goat | Sigma-Aldrich, St. Louis, MO, USA |
Human albumin | 66 | 6.8 | Goat | OEM Concepts, Saco, ME, USA |
Plasminogen | 90 | 7.3 | Goat | Cedarlane Laboratories, ON, CAN |
Complement factor 3 (C3) | 185 | 6.4 | Goat | Calbiochem, Gibbstown, NJ, USA |
Factor XII | 80 | 7.7 | Goat | Cedarlane Laboratories, ON, CAN |
Factor XI | 160 | 8.1 | Goat | Cedarlane Laboratories, ON, CAN |
Apolipoprotein A1 | 28 | 7.1 | Goat | Sigma-Aldrich, St. Louis, MO, USA |
Transferrin | 77 | 7.1 | Goat | Sigma-Aldrich, St. Louis, MO, USA |
α2-Macroglobulin | 718 | 6.4 | Goat | Sigma-Aldrich, St. Louis, MO, USA |