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Biointerphases

Journal for Biophysical Chemistry

Fig. 5 | Biointerphases

Fig. 5

From: Micrometer and Nanometer Scale Photopatterning of Proteins on Glass Surfaces by Photo-degradation of Films Formed from Oligo(Ethylene Glycol) Terminated Silanes

Fig. 5

a Fluorescence microscopy image of a photopatterned OEG-silane film following immersion in a solution of Neutravidin nanoparticles. b Tapping mode AFM topographical image of a sample similar to the one shown in a. z-scale range (dark to bright) 0–60 nm. c Fluorescence micrograph of a photodegraded OEG-silane film following immersion in a solution of Alexa fluor 532-labeled streptavidin. d Tapping mode AFM topographical image of a pattern formed by UV exposure through a mask, followed by attachment of AB-NTA, complexation with Ni2+ and incubation in a solution of His-tagged YFP. e, f Confocal fluorescence microscopy images of similar samples to the one shown in d

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