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Journal for Biophysical Chemistry

Fig. 6 | Biointerphases

Fig. 6

From: Investigations on the Secondary Structure of Polypeptide Chains in Polyelectrolyte Multilayers and their Effect on the Adhesion and Spreading of Osteoblasts

Fig. 6

Cell biological analyses on multilayers constructed from single and multiple polyanions with respect to the conformation of polypeptide chains in the film expressed as the percentage of conformation B. All PEM films were prepared with a top layer of the respective polyanion (CS or HEP). a, b Cell morphology of SaOs osteoblasts cultured on the films for 4 days (red actin cytoskelleton stained with a phalloidin conjugate, blue nuclei stained with Hoechst 33258, scale bar 20 μm). c Cell count on samples that were incubated with osteoblasts for 7 days referenced to uncoated glass controls (data are presented as mean ± SD for nine samples per modification in three independent experiments, p values for significantly different pairs are indicated)

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