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Biointerphases

Journal for Biophysical Chemistry

Fig. 7 | Biointerphases

Fig. 7

From: Investigations on the Secondary Structure of Polypeptide Chains in Polyelectrolyte Multilayers and their Effect on the Adhesion and Spreading of Osteoblasts

Fig. 7

Characterization of patterned PEM films that were prepared by incubating a droplet of PGA solution onto a preassembled (PLL-CS)20 multilayer on glass. a FTIR-ATR microscopy results are presented as integration over the wave number range from 1,600 to 1,635 cm−1. Color scale from white to grey indicates an increase in conformation B (0.0 to 0.6 arbitrary integration units, image size 1.4 mm × 1.5 mm). b Colloidal force microscopy map that indicates an increasing stiffness of the PGA treated film area (color scale white = 47 pN/nm to black = 68 pN/nm, image size (100 μm)2). c SEM image of osteoblasts that were incubated on the patterned PEM films for 7 days (original magnification ×500, scale bar 10 μm). A segment of the circular PGA treated area is depicted in the upper right corner of each panel

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