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Biointerphases

Journal for Biophysical Chemistry

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Live cell adhesion assay with attenuated total reflection infrared spectroscopy

Biointerphases volume 2, pages 1–5 (2007)Cite this article

Abstract

Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection ATR infrared IR spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability ofATR IR spectroscopy for live cell adhesion assays.

References

  1. D. Naumann, Infrared Phys. 24, 233 1984.

    Article  CAS  Google Scholar 

  2. D. G. Cameron, A. Martin, D. J. Moffatt, and H. H. Mantsch, Biochemistry 24, 4355 1985.

    Article  CAS  Google Scholar 

  3. H. N. Holman, M. C. Martin, and W. R. McKinney, J. Biol. Phys. 29, 275 2003.

    Article  CAS  Google Scholar 

  4. G. D. Sockalingum, W. Bouhedja, P. Pina, P. Allouch, C. Bloy, and M. Manfait, Cell. Mol. Biol. Paris 44, 261 1998.

    CAS  Google Scholar 

  5. T. B. Hutson, M. L. Mitchell, J. T. Keller, D. J. Long, and M. J. W. Chang, Anal. Biochem. 174, 415 1988.

    Article  CAS  Google Scholar 

  6. M. K. Alam, J. A. Timlin, L. E. Martin, D. Williams, C. R. Lyons, K. Garrison, and B. Hjelle, Vib. Spectrosc. 34, 3 2004.

    Article  CAS  Google Scholar 

  7. Y. Maréchal, J. Phys. Chem. 97, 2846 1993.

    Article  Google Scholar 

  8. K. Maquelin, L.-P. Choo-Smith, C. Kirschner, N. A. Ngo-Thi, D. Naumann, and G. J. Puppels, in Handbook of Vibrational Spectroscopy, edited by J. M. Chalmers and P. R. Griffiths (Wiley, New York, 2002, Vol. 5, pp. 3308–3334.

    Google Scholar 

  9. S. J. Parikh and J. Chorover, Langmuir 22, 8492 2006.

    Article  CAS  Google Scholar 

  10. See EPAPS Document No. E-BJIOBN-2-003701 for a table listing the band assignments according to the literature. This document can be reached via a direct link in the online article’s HTML reference section or via the EPAPS homepage http://www.aip.org/pubservs/epaps.html.

  11. L. A. Thomson, F. C. Law, N. Rushton, and J. Franks, Biomaterials 12, 37 1991.

    Article  CAS  Google Scholar 

  12. J. A. Cooper, J. Cell Biol. 105, 1473 1987.

    Article  CAS  Google Scholar 

  13. K. P. Ishida and P. R. Griffiths, Appl. Spectrosc. 47, 584 1993.

    Article  CAS  Google Scholar 

  14. N. Jamin, P. Dumas, J. Moncuit, W.-H. Fridman, J.-L. Teillaud, G. L. Carr, and G. P. Williams, Proc. Natl. Acad. Sci. U.S.A. 95, 4837 1998.

    Article  CAS  Google Scholar 

  15. H. N. Holman, M. C. Martin, E. A. Blakely, K. Bjornstad, and W. R. McKinney, Biopolymers 57, 329 2000.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute for Molecular Biophysics, 600 Main Street, 04609, Bar Harbor, Maine

    Martin Schmidt, Tobias Wolfram & Michael Grunze

  2. Angewandte Physikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Martin Schmidt

  3. Biophysikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Tobias Wolfram

  4. Max-Planck-Institut für Metallforschung, Abteilung Neue Materialien und Biosysteme, Heisenbergstraße 3, 70569, Stuttgart, Germany

    Tobias Wolfram

  5. Max-Planck-Institut für Kolloid-und Grenzflächenforschung, Abteilung Biomaterialien, Am Mühlenberg, 14424, Potsdam, Germany

    Monika Rumpler

  6. Department of Chemistry, University of Maine, 04469, Orono, Maine

    Carl P. Tripp

  7. Laboratory for Surface Science and Technology, University of Maine, 04469, Orono, Maine

    Carl P. Tripp

  8. Angewandte Physikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Michael Grunze

Authors
  1. Martin Schmidt
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  2. Tobias Wolfram
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  3. Monika Rumpler
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  4. Carl P. Tripp
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  5. Michael Grunze
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Correspondence to Martin Schmidt.

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Schmidt, M., Wolfram, T., Rumpler, M. et al. Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases 2, 1–5 (2007). https://doi.org/10.1116/1.2710336

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  • DOI: https://doi.org/10.1116/1.2710336