Biointerphases

Journal for Biophysical Chemistry

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Open Access

Live cell adhesion assay with attenuated total reflection infrared spectroscopy

  • Martin Schmidt1, 2Email author,
  • Tobias Wolfram1, 3, 4,
  • Monika Rumpler5,
  • Carl P. Tripp6, 7 and
  • Michael Grunze1, 8
Biointerphases2:20100001

https://doi.org/10.1116/1.2710336

Received: 18 December 2006

Accepted: 26 January 2007

Abstract

Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection ATR infrared IR spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability ofATR IR spectroscopy for live cell adhesion assays.