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Biointerphases

Journal for Biophysical Chemistry

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Live cell adhesion assay with attenuated total reflection infrared spectroscopy

Biointerphases volume 2, pages 1–5 (2007)Cite this article

Abstract

Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection ATR infrared IR spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability ofATR IR spectroscopy for live cell adhesion assays.

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Authors and Affiliations

  1. Institute for Molecular Biophysics, 600 Main Street, 04609, Bar Harbor, Maine

    Martin Schmidt, Tobias Wolfram & Michael Grunze

  2. Angewandte Physikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Martin Schmidt

  3. Biophysikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Tobias Wolfram

  4. Max-Planck-Institut für Metallforschung, Abteilung Neue Materialien und Biosysteme, Heisenbergstraße 3, 70569, Stuttgart, Germany

    Tobias Wolfram

  5. Max-Planck-Institut für Kolloid-und Grenzflächenforschung, Abteilung Biomaterialien, Am Mühlenberg, 14424, Potsdam, Germany

    Monika Rumpler

  6. Department of Chemistry, University of Maine, 04469, Orono, Maine

    Carl P. Tripp

  7. Laboratory for Surface Science and Technology, University of Maine, 04469, Orono, Maine

    Carl P. Tripp

  8. Angewandte Physikalische Chemie, Universität Heidelberg, Im Neuenheimer Feld 253, 69120, Heidelberg, Germany

    Michael Grunze

Authors
  1. Martin Schmidt
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  2. Tobias Wolfram
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  3. Monika Rumpler
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  4. Carl P. Tripp
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  5. Michael Grunze
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Correspondence to Martin Schmidt.

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Schmidt, M., Wolfram, T., Rumpler, M. et al. Live cell adhesion assay with attenuated total reflection infrared spectroscopy. Biointerphases 2, 1–5 (2007). https://doi.org/10.1116/1.2710336

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  • DOI: https://doi.org/10.1116/1.2710336