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Direct observation of interaction between proteins and blood-compatible polymer surfaces
Biointerphases volume 2, pages 119–125 (2007)
Abstract
The adhesion force between blood-compatible polymer (poly(2-methoxyethyl acrylate: PMEA) and proteins (fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface showed almost no adhesion to native protein molecules, whereas non-blood-compatible poly (n-butyl acrylate): PBA strongly adhered to proteins. Interestingly, adhesion did appear between PMEA and proteins when the proteins were denatured. In all cases, these trends were not affected by the conditions of the solution. Combining the results with previous reports, the authors conclude that interfacial water molecules play a critical role in the protein resistance of PMEA.
References
T. Tsuruta, Adv. Polym. Sci. 126, 1 (1996).
M. Tanaka, T. Motomura, M. Kawada, T. Anzai, Y. Kasori, T. Shiroya, K. Shimura, M. Onishi, and A. Mochizuki, Biomaterials 21, 1471 (2000).
H. Kitano, K. Ichikawa, M. Fukuda, A. Mochizuki, and M. Tanaka, J. Colloid Interface Sci. 242, 133 (2001) M. Tanaka, Biomed. Mater. Eng. 14, 427 (2004).
M. Tanaka, Kobunshi Rombunshu 60, 415 (2003); M. Tanaka, A. Mochizuki, N. Ishii, T. Motomura, and T. Hatakeyama, Biomacromolecules 3, 36 (2002); M. Tanaka, T. Motomura, N. Ishii, K. Shimura, M. Onishi, A. Mochizuki, and T. Hatakeyama, Polym. Int. 49, 1709 (2000).
M. Tanaka and A. Mochizuki, J. Biomed. Mater. Res. A 68A, 684 (2004).
M. Tanaka, A. Mochizuki, T. Motomura, K. Shimura, M. Onishi, and Y. Okahata, Colloids Surf. A Physicochem. Eng. Aspect. 193, 145 (2001); M. Tanaka, A. Mochizuki, T. Shiroya, T. Motomura, K. Shimura, M. Onishi, and Y. Okahata, ibid. 203, 195 (2002).
D. Baykut, F. Bernet, J. Wehrle, K. Weichelt, P. Schwartz, and H. R. Zerkowski, Eur. J. Med. Res. 6, 297 (2001).
M. Ide, T. Mori, K. Ichikawa, H. Kitano, M. Tanaka, A. Mochizuki, H. Oshiyama, and W. Mizuno, Langmuir 19, 429 (2003).
S. Morita, M. Tanaka, and Y. Ozaki, Langmuir 23, 3750 (2007).
J. Israelachvili, Intermolecular and Surface Forces (Academic, London, 1992).
D. Leckband and J. Israelachvili, Q. Rev. Biophys. 34, 105 (2001).
H. J. Butt, Biophys. J. 60, 1438 (1991); 60, 1438 (1991).
J. L. Hutter and J. Bechhoefer, Rev. Sci. Instrum. 64, 1868 (1993).
K. Feldman, G. Haehner, N. D. Spencer, P. Harder, and M. Grunze, J. Am. Chem. Soc. 121, 10134 (1999).
S. Tunc, M. F. Maitz, G. Steiner, L. Vazquez, M. T. Pham, and R. Salzer, Colloids Surf. B Biointerfaces 42, 219 (2005).
C. E. Giacomelli and W. Norde, J. Colloid Interface Sci. 233, 234 (2001).
T. A. Horbett, B. D. Ratner, A. S. Hoffman, F. J. Schoen, and J. E. Lemons, Biomaterials Science (Academic, San Diego, 1996); T. A. Horbett and J. L. Brash, Proteins at Interfaces II. Fundamentals and Applications (American Chemical Society, Washington, DC, 1995).
R. J. M. Peula-Garcia, R. Hidalgo-Alvarez, and F. de las Nieves, Colloid Polym. Sci. 275, 198 (1997).
A. Henschen, J. McDonagh, R. F. A. Zwaal, and H. C. Hemker, Blood Coagulation (Elsevier, Amsterdam, 1986).
I. Larson and R. J. Pugh, Langmuir 14, 5676 (1998).
Y. H. M. Chan, R. Schweiss, C. Werner, and M. Grunze, Langmuir 19, 7380 (2003); R. Schweiss, P. B. Welzel, C. Werner, and W. Knoll, Colloids Surf. A Physicochem. Eng. Aspect. 195, 97 (2001).
S. Herrwerth, W. Eck, S. Reinhardt, and M. Grunze, J. Am. Chem. Soc. 125, 9359 (2003). or]23 See EPAPS Document No. E-BJIOBN-2-001704 for the adsorption kinetics of BSA onto the PBA surface measured by QCM. This document can be reached through a direct link in the online article’s HTML reference section or via the EPAPS homepage (http://www.aip.org/pubservs/epaps.html).
S. I. Jeon, J. H. Lee, J. D. Andrade, and P. G. Degennes, J. Colloid Interface Sci. 142, 149 (1991); T. McPherson, A. Kidane, I. Szleifer, and K. Park, Langmuir 14, 176 (1998).
K. Nishinari, M. Watase, and T. Hatakeyama, Colloid Polym. Sci. 275, 1078 (1997).
K. Kawai, T. Suzuki, and M. Oguni, Biophys. J. 90, 3732 (2006).
M. Nakamura, H. Uematsu, and H. Shiomi, J. Ceram. Soc. Jpn. 106, 103 (1998).
A. J. Pertsin and M. Grunze, Langmuir 16, 8829 (2000); J. Zheng, L. Y. Li, H. K. Tsao, Y. J. Sheng, S. F. Chen, and S. Y. Jiang, Biophys. J. 89, 158 (2005); J. Zheng, L. Y. Li, S. F. Chen, and S. Y. Jiang, Langmuir 20, 8931 (2004); J. G. Archambault and J. L. Brash, Colloids Surf. B Biointerfaces 33, 111 (2004).
S. F. Chen, F. C. Yu, Q. M. Yu, Y. He, and S. Y. Jiang, Langmuir 22, 8186 (2006).
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Hayashi, T., Tanaka, M., Yamamoto, S. et al. Direct observation of interaction between proteins and blood-compatible polymer surfaces. Biointerphases 2, 119–125 (2007). https://doi.org/10.1116/1.2794712
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DOI: https://doi.org/10.1116/1.2794712