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Journal for Biophysical Chemistry

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Direct observation of interaction between proteins and blood-compatible polymer surfaces


The adhesion force between blood-compatible polymer (poly(2-methoxyethyl acrylate: PMEA) and proteins (fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface showed almost no adhesion to native protein molecules, whereas non-blood-compatible poly (n-butyl acrylate): PBA strongly adhered to proteins. Interestingly, adhesion did appear between PMEA and proteins when the proteins were denatured. In all cases, these trends were not affected by the conditions of the solution. Combining the results with previous reports, the authors conclude that interfacial water molecules play a critical role in the protein resistance of PMEA.


  1. 1

    T. Tsuruta, Adv. Polym. Sci. 126, 1 (1996).

    CAS  Google Scholar 

  2. 2

    M. Tanaka, T. Motomura, M. Kawada, T. Anzai, Y. Kasori, T. Shiroya, K. Shimura, M. Onishi, and A. Mochizuki, Biomaterials 21, 1471 (2000).

    Article  CAS  Google Scholar 

  3. 3

    H. Kitano, K. Ichikawa, M. Fukuda, A. Mochizuki, and M. Tanaka, J. Colloid Interface Sci. 242, 133 (2001) M. Tanaka, Biomed. Mater. Eng. 14, 427 (2004).

    Article  CAS  Google Scholar 

  4. 4

    M. Tanaka, Kobunshi Rombunshu 60, 415 (2003); M. Tanaka, A. Mochizuki, N. Ishii, T. Motomura, and T. Hatakeyama, Biomacromolecules 3, 36 (2002); M. Tanaka, T. Motomura, N. Ishii, K. Shimura, M. Onishi, A. Mochizuki, and T. Hatakeyama, Polym. Int. 49, 1709 (2000).

    Article  CAS  Google Scholar 

  5. 5

    M. Tanaka and A. Mochizuki, J. Biomed. Mater. Res. A 68A, 684 (2004).

    Article  Google Scholar 

  6. 6

    M. Tanaka, A. Mochizuki, T. Motomura, K. Shimura, M. Onishi, and Y. Okahata, Colloids Surf. A Physicochem. Eng. Aspect. 193, 145 (2001); M. Tanaka, A. Mochizuki, T. Shiroya, T. Motomura, K. Shimura, M. Onishi, and Y. Okahata, ibid. 203, 195 (2002).

    Article  CAS  Google Scholar 

  7. 7

    D. Baykut, F. Bernet, J. Wehrle, K. Weichelt, P. Schwartz, and H. R. Zerkowski, Eur. J. Med. Res. 6, 297 (2001).

    CAS  Google Scholar 

  8. 8

    M. Ide, T. Mori, K. Ichikawa, H. Kitano, M. Tanaka, A. Mochizuki, H. Oshiyama, and W. Mizuno, Langmuir 19, 429 (2003).

    Article  CAS  Google Scholar 

  9. 9

    S. Morita, M. Tanaka, and Y. Ozaki, Langmuir 23, 3750 (2007).

    Article  CAS  Google Scholar 

  10. 10

    J. Israelachvili, Intermolecular and Surface Forces (Academic, London, 1992).

    Google Scholar 

  11. 11

    D. Leckband and J. Israelachvili, Q. Rev. Biophys. 34, 105 (2001).

    Article  CAS  Google Scholar 

  12. 12

    H. J. Butt, Biophys. J. 60, 1438 (1991); 60, 1438 (1991).

    Article  CAS  Google Scholar 

  13. 13

    J. L. Hutter and J. Bechhoefer, Rev. Sci. Instrum. 64, 1868 (1993).

    Article  CAS  Google Scholar 

  14. 14

    K. Feldman, G. Haehner, N. D. Spencer, P. Harder, and M. Grunze, J. Am. Chem. Soc. 121, 10134 (1999).

    Article  CAS  Google Scholar 

  15. 15

    S. Tunc, M. F. Maitz, G. Steiner, L. Vazquez, M. T. Pham, and R. Salzer, Colloids Surf. B Biointerfaces 42, 219 (2005).

    Article  CAS  Google Scholar 

  16. 16

    C. E. Giacomelli and W. Norde, J. Colloid Interface Sci. 233, 234 (2001).

    Article  CAS  Google Scholar 

  17. 17

    T. A. Horbett, B. D. Ratner, A. S. Hoffman, F. J. Schoen, and J. E. Lemons, Biomaterials Science (Academic, San Diego, 1996); T. A. Horbett and J. L. Brash, Proteins at Interfaces II. Fundamentals and Applications (American Chemical Society, Washington, DC, 1995).

    Google Scholar 

  18. 18

    R. J. M. Peula-Garcia, R. Hidalgo-Alvarez, and F. de las Nieves, Colloid Polym. Sci. 275, 198 (1997).

    Article  CAS  Google Scholar 

  19. 19

    A. Henschen, J. McDonagh, R. F. A. Zwaal, and H. C. Hemker, Blood Coagulation (Elsevier, Amsterdam, 1986).

    Google Scholar 

  20. 20

    I. Larson and R. J. Pugh, Langmuir 14, 5676 (1998).

    Article  CAS  Google Scholar 

  21. 21

    Y. H. M. Chan, R. Schweiss, C. Werner, and M. Grunze, Langmuir 19, 7380 (2003); R. Schweiss, P. B. Welzel, C. Werner, and W. Knoll, Colloids Surf. A Physicochem. Eng. Aspect. 195, 97 (2001).

    Article  CAS  Google Scholar 

  22. 22

    S. Herrwerth, W. Eck, S. Reinhardt, and M. Grunze, J. Am. Chem. Soc. 125, 9359 (2003). or]23 See EPAPS Document No. E-BJIOBN-2-001704 for the adsorption kinetics of BSA onto the PBA surface measured by QCM. This document can be reached through a direct link in the online article’s HTML reference section or via the EPAPS homepage (

    Article  CAS  Google Scholar 

  23. 24

    S. I. Jeon, J. H. Lee, J. D. Andrade, and P. G. Degennes, J. Colloid Interface Sci. 142, 149 (1991); T. McPherson, A. Kidane, I. Szleifer, and K. Park, Langmuir 14, 176 (1998).

    Article  CAS  Google Scholar 

  24. 25

    K. Nishinari, M. Watase, and T. Hatakeyama, Colloid Polym. Sci. 275, 1078 (1997).

    Article  CAS  Google Scholar 

  25. 26

    K. Kawai, T. Suzuki, and M. Oguni, Biophys. J. 90, 3732 (2006).

    Article  CAS  Google Scholar 

  26. 27

    M. Nakamura, H. Uematsu, and H. Shiomi, J. Ceram. Soc. Jpn. 106, 103 (1998).

    Article  CAS  Google Scholar 

  27. 28

    A. J. Pertsin and M. Grunze, Langmuir 16, 8829 (2000); J. Zheng, L. Y. Li, H. K. Tsao, Y. J. Sheng, S. F. Chen, and S. Y. Jiang, Biophys. J. 89, 158 (2005); J. Zheng, L. Y. Li, S. F. Chen, and S. Y. Jiang, Langmuir 20, 8931 (2004); J. G. Archambault and J. L. Brash, Colloids Surf. B Biointerfaces 33, 111 (2004).

    Article  CAS  Google Scholar 

  28. 29

    S. F. Chen, F. C. Yu, Q. M. Yu, Y. He, and S. Y. Jiang, Langmuir 22, 8186 (2006).

    Article  CAS  Google Scholar 

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Correspondence to Tomohiro Hayashi.

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Hayashi, T., Tanaka, M., Yamamoto, S. et al. Direct observation of interaction between proteins and blood-compatible polymer surfaces. Biointerphases 2, 119–125 (2007).

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