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Biointerphases

Journal for Biophysical Chemistry

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Creation of a mixed poly(ethylene glycol) tethered-chain surface for preventing the nonspecific adsorption of proteins and peptides

Biointerphases volume 2, pages 126–130 (2007)Cite this article

Abstract

Using a heterotelechelic poly(ethylene glycol) (PEG) possessing a mercapto group at one end and an acetal group at the other end (acetal-PEG-SH), the authors constructed a reactive PEG tethered-chain surface on a surface plasmon sensor (SPR) gold chip for biosensing with high sensitivity. Nonspecific bovine serum albumin adsorption on the PEG tethered-chain surface was significantly influenced by the density of the PEG chain, and was almost completely suppressed by increasing the PEG density through the repetitive treatment of the chip surface with acetal-PEG-SH. The PEG density was increased even more by adding an underbrushed layer made of shorter-PEG-SH-chain molecules (2 kDa, hereafter 2k) to the surface made of longer-PEG-SH-chain molecules (5 kDa, hereafter 5k). SPR measurement then gave an estimate of the adsorption of a series of proteins with varying sizes and isoelectric points on the PEG chain surface having an underbrushed layer (PEG5k/2k surface). As compared to other SPR surfaces, viz., a commercial carboxymethyl dextran and conventional PEG5k tethered-chain surface without an underbrushed layer, the mixed PEG5k/2k surface showed almost complete inhibition of the nonspecific adsorption not only of high-molecular-weight proteins but also of low-molecular-weight peptides.

References

  1. Y. Mori, S. Nagaoka, H. Takiuchi, T. Kikuchi, N. Noguchi, H. Tanzawa, and Y. Noishiki, Trans. Am. Soc. Artif. Intern. Organs 28, 459 (1982).

    CAS  Google Scholar 

  2. K. Bergdtröm, E. Österberg, K. Holmberg, A. S. Hoffman, T. P. Schuman, A. Kozlowski, and J. M. Harris, J. Biomater. Sci. Polym. Ed. 6, 123 (1994).

    Article  Google Scholar 

  3. Poly(ethylene glycol) Chemistry: Biotechnical and Biomedical Applications edited by J. M. Harris (Plenum,New York, 1992).

    Google Scholar 

  4. Hydrophilic Polymers: Performance with Environmental Acceptance, edited by J. E. Glass (American Chemistry Society, Washington, DC, 1996).

    Google Scholar 

  5. Y. S. Lin, V. Hlady, and C. G. Golander, Colloids Surf., B 3, 49 1994.

  6. N. P. Huang, J. Voeroes, S. M. De Paul, M. Textor, and N. D. Spencer, Langmuir 18, 220 (2002).

    Article  CAS  Google Scholar 

  7. S. Pasche, S. M. De Paul, J. Voeroes, N. D. Spencer, and M. Textor, Langmuir 19, 9216 (2003).

    Article  CAS  Google Scholar 

  8. N. Xia, Y. Hu, D. W. Grainger, and D. G. Castner, Langmuir 18, 3255 (2002).

    Article  CAS  Google Scholar 

  9. H. Otsuka, Y. Nagasaki, and K. Kataoka, Langmuir 20, 11285 (2004).

    Article  CAS  Google Scholar 

  10. S. I. Jeon, J. H. Lee, J. D. Andrade, and P. G. de Gennes, J. Colloid Interface Sci. 142, 159 (1991).

    Article  CAS  Google Scholar 

  11. N. L. Abbott, D. Blankschtein, and T. A. Hatton, Macromolecules 25, 5192 (1992).

    Article  CAS  Google Scholar 

  12. Y. Akiyama, H. Otsuka, Y. Nagasaki, M. Kato, and K. Kataoka, Bioconjug. Chem. 11, 947 (2000).

    Article  CAS  Google Scholar 

  13. H. Otsuka, Y. Akiyama, Y. Nagasaki, and K. Kataoka, J. Am. Chem. Soc. 123, 8226 (2001).

    Article  CAS  Google Scholar 

  14. K. Uchida, H. Otsuka, M. Kaneko, K. Kataoka, and Y. Nagasaki, Anal. Chem. 77, 1075 (2005).

    Article  CAS  Google Scholar 

  15. B. Liedberg, C. Nylander, and I. Lungström, Sens. Actuators 4, 299 (1983).

    Article  CAS  Google Scholar 

  16. J. Melendez, R. Carr, D. U. Barholomew, K. Kukanskis, J. Elkind, S. Yee, C. Furlong, and R. Woodbury, Sens. Actuators, B 35, 212 (1996).

    Article  Google Scholar 

  17. R. J. Whelan, T. Wohland, L. Neumann, B. Huang, B. Kobilka, and R. N. Zare, Anal. Chem. 74, 4570 (2002).

    Article  CAS  Google Scholar 

  18. L. G. Fägerstam, Å. Frosell-Karlsson, R. Karlsson, B. Persson, and I. Rönnberg, J. Chromatogr. 597, 397 (1992).

    Article  Google Scholar 

  19. R. Cush, J. M. Cronin, W. J. Stewart, C. H. Maule, J. Molloy, and N. J. Goddard, Biosens. Bioelectron. 8, 347 (1993).

    Article  CAS  Google Scholar 

  20. M. Malmqvist and R. Karlsson, Curr. Opin. Chem. Biol. 1, 378 (1997).

    Article  CAS  Google Scholar 

  21. T. Natsume, H. Nakayama, Ö. Jansson, T. Isobe, K. Takio, and K. Mikoshiba, Anal. Chem. 72, 4193 (2000).

    Article  CAS  Google Scholar 

  22. M. J. Gómara, G. Ercilla, M. A. Alsina, and I. Haro, J. Immunol. Methods 246, 13 (2000).

    Article  Google Scholar 

  23. R. Nakamura, H. Muguruma, K. Ikebukuro, S. Sasaki, R. Nagata, I. Karube, and H. Pedersen, Anal. Chem. 69, 4649 1997.

    Article  CAS  Google Scholar 

  24. F. F. Bier, F. Kleinjung, and F. W. Scheller, Sens. Actuators, B 38, 78 (1997).

    Article  Google Scholar 

  25. C. Williams and T. A. Addona, Trends Biotechnol. 18, 45 (2000).

    Article  CAS  Google Scholar 

  26. J. M. McDonnell, Curr. Opin. Chem. Biol. 5, 572 (2001).

    Article  CAS  Google Scholar 

  27. F. Yu and W. Knoll, Anal. Chem. 76, 1971 (2004).

    Article  CAS  Google Scholar 

  28. D. J. O’Shannessy, M. Brigham-Burke, and K. Peck, Anal. Biochem. 205, 132 (1992).

    Article  Google Scholar 

  29. E. Stenberg, B. Persson, H. Roos, and C. Urbaniczky, J. Colloid Interface Sci. 143, 513 (1991). or]30 See EPAPS Document No. E-BJIOBN-2-003704 for details on using radiolabeling protein, the absolute amount of adsorbed protein on a PEG tethered chain surface was estimated. This document can be reached through a direct link in the online article’s HTML reference section or via the EPAPS homepage (http://www.aip.org/pubservs/epaps.html).

    Article  CAS  Google Scholar 

  30. W. T. E. Bosker, P. A. Iakovlev, and W. Norde, J. Colloid Interface Sci. 286, 496 (2005).

    Article  CAS  Google Scholar 

  31. T. Ishii, H. Otsuka, K. Kataoka, and Y. Nagasaki, Langmuir 20, 561 (2004).

    Article  CAS  Google Scholar 

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Author notes

  1. Katsumi Uchida & Hidenori Otsuka

    Present address: Department of Applied Chemistry, Tokyo University of Science, 12-1 Funagawara-cho, Ichigaya, 162-0826, Shinjuku-ku, Tokyo, Japan

Authors and Affiliations

  1. Department of Materials Science, Tokyo University of Science, 2641 Yamazaki, 270-8510, Noda-shi, Chiba, Japan

    Katsumi Uchida

  2. Graduate School of Pure and Applied Sciences, University of Tsukuba, Ten-noudai 1-1-1, 305-8573, Tsukuba, Ibaraki, Japan

    Yuki Hoshino, Atsushi Tamura & Keitaro Yoshimoto

  3. Department of Pharmacy, Tokyo University of Science, 2641 Yamazaki, 270-8510, Noda-shi, Chiba, Japan

    Shuji Kojima & Keichiro Yamashita

  4. Department of Applied Chemistry, Tokyo University of Science, 12-1 Funagawara-cho, Ichigaya, 162-0826, Shinjuku-ku, Tokyo, Japan

    Ichiro Yamanaka

  5. Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, 113-8656, Bunkyo-ku, Tokyo, Japan

    Hidenori Otsuka & Kazunori Kataoka

  6. Graduate School of Pure and Applied Sciences and Tsukuba Research Center for Interdisciplinary Material Sciences, University of Tsukuba, Ten-noudai 1-1-1, 305-8573, Tsukuba, Ibaraki, Japan

    Yukio Nagasaki

Authors
  1. Katsumi Uchida
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  2. Yuki Hoshino
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  3. Atsushi Tamura
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  4. Keitaro Yoshimoto
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  5. Shuji Kojima
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  6. Keichiro Yamashita
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  7. Ichiro Yamanaka
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  8. Hidenori Otsuka
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  9. Kazunori Kataoka
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  10. Yukio Nagasaki
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Corresponding author

Correspondence to Yukio Nagasaki.

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Uchida, K., Hoshino, Y., Tamura, A. et al. Creation of a mixed poly(ethylene glycol) tethered-chain surface for preventing the nonspecific adsorption of proteins and peptides. Biointerphases 2, 126–130 (2007). https://doi.org/10.1116/1.2800754

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  • DOI: https://doi.org/10.1116/1.2800754