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Biointerphases

Journal for Biophysical Chemistry

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Selective immobilization of biomolecules onto an activated polymeric adlayer

Biointerphases volume 2, pages 136–142 (2007)Cite this article

Abstract

The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent attachment of biomolecules. For this purpose, a random copolymer, poly(PEGMA-r-NAS), was synthesized by radical polymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and N-acryloxysuccinimide (NAS). In the first step, the polymeric adlayer was formed onto amine-terminated self-assembled monolayers (SAMs) on gold through covalent bond formation between reactive N-hydroxysuccinimide (NHS) ester of the copolymer and the amine of the SAMs. In the second step, amine-bearing biotin as a model biomolecule was covalently attached onto the polymeric adlayer that still contained unreacted NHS esters. The degrees of the binding sensitivity for a target protein and the nonspecific binding for four model proteins on the biotinylated polymeric adlayer were examined by surface plasmon resonance spectroscopy. Finally, the specific immobilization of rhodamin (TRITC)-conjugated streptavidin on the biotinylated polymeric adlayer was achieved by a simple microcontact printing technique, resulting in well-defined patterns of the protein.

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Authors and Affiliations

  1. Department of Chemistry and School of Molecular Science (BK21), Center for Molecular Design and Synthesis, Korea Advanced Institute of Science and Technology (KAIST), 305-701, Daejeon, Korea

    Bong Soo Lee & Insung S. Choi

  2. Research Center for Biomolecular Nanotechnology, Department of Life Science, Gwangju Institute of Science and Technology (GIST), 500-712, Gwangju, Korea

    Sangjin Park & Sangyong Jon

  3. Glycomics Team, Korea Basic Science Institute (KBSI), 305-333, Daejeon, Korea

    Kyung-Bok Lee

Authors
  1. Bong Soo Lee
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  2. Sangjin Park
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  3. Kyung-Bok Lee
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  4. Sangyong Jon
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  5. Insung S. Choi
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Correspondence to Sangyong Jon or Insung S. Choi.

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Lee, B.S., Park, S., Lee, KB. et al. Selective immobilization of biomolecules onto an activated polymeric adlayer. Biointerphases 2, 136–142 (2007). https://doi.org/10.1116/1.2801974

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  • DOI: https://doi.org/10.1116/1.2801974