Skip to main content

Journal for Biophysical Chemistry

Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes

Abstract

Streaming potential/current measurements for the characterization of charge formation processes at solid/liquid interfaces were combined with reflectometric interference spectroscopy. The simultaneous determination of electrosurface characteristics and the optical thickness of interfacial layers provides information on structural variations of adsorbed or covalently bound polymers and on charge dependent adsorption and desorption phenomena at solid/liquid interfaces. To demonstrate the potentialities of this extended approach for biointerfacial studies the authors report a series of experiments on the adsorption of the plasma protein fibrinogen at poly(octadecene-alt-maleic acid) thin films.

References

  1. D. Myers, Surfaces, Interfaces, and Colloids, 2nd ed. (Wiley, New York, 1999).

    Book  Google Scholar 

  2. A. Härtl, S. Nowy, R. Zimmermann, C. Werner, D. Horinek, R. Netz, and M. Stutzmann, J. Am. Chem. Soc. 129, 1287 (2007).

    Article  Google Scholar 

  3. C. Dicke and G. Hähner, J. Am. Chem. Soc. 124, 12619 (2002).

    Article  CAS  Google Scholar 

  4. R. Zimmermann, S. S. Dukhin, and C. Werner, J. Phys. Chem. B 105, 8544 (2001).

    Article  CAS  Google Scholar 

  5. H.-J. Jacobasch, K. Grundke, S. Schneider, and F. Simon, J. Adhes. 48, 57 (1995).

    Article  CAS  Google Scholar 

  6. A. Bismarck, M. E. Kumru, and J. Springer, J. Colloid Interface Sci. 217, 377 (1999).

    Article  CAS  Google Scholar 

  7. X. Liu, W. Farmerie, S. Schuster, and W. Tan, Anal. Biochem. 283, 56 (2000).

    Article  CAS  Google Scholar 

  8. A. Baerga-Ortiz, A. R. Rezaie, and E. A. Komives, J. Mol. Biol. 296, 651 (2000).

    Article  CAS  Google Scholar 

  9. R. van Reis, J. M. Brake, J. Charkoudian, D. B. Burns, and A. L. Zydney, J. Membr. Sci. 159, 133 (1999).

    Article  Google Scholar 

  10. M. Ernst, A. Bismarck, J. Springer, and M. Jekel, J. Membr. Sci. 165, 251 (2000).

    Article  CAS  Google Scholar 

  11. R. Zimmermann, T. Kratzmüller, D. Erickson, D. Li, D. H.-G. Braun, and C. Werner, Langmuir 20, 2369 (2004).

    Article  CAS  Google Scholar 

  12. R. Hidalgo-Álvarez, F. J. de las Nieves, A. J. van der Linde, and B. H. Bijsterbosch, Colloid Polym. Sci. 267, 853 (1989).

    Article  Google Scholar 

  13. Á. V. Delgado and F. J. Arroyo, in Interfacial Electrokinetics and Electrophoresis, edited by Á. V. Delgado (Marcel Dekker, New York, 2001), pp. 1–54.

    Google Scholar 

  14. J. Lyklema, Fundamentals of Colloid and Interface Science (Academic, London, 1991), Vol. II.

    Google Scholar 

  15. C. Werner, R. Zimmermann, and T. Kratzmüller, Colloids Surf., A 192, 205 (2001).

    Article  CAS  Google Scholar 

  16. W. Norde and E. Rouwendal, J. Colloid Interface Sci. 139, 169 (1990).

    Article  CAS  Google Scholar 

  17. M. Zembala and P. Déjardin, Colloids Surf., B 3, 119 (1994).

    Article  CAS  Google Scholar 

  18. H. Shirahama, J. Lyklema, and W. Norde, J. Colloid Interface Sci. 139, 177 (1990).

    Article  CAS  Google Scholar 

  19. C. Werner, H. Körber, R. Zimmermann, S. S. Dukhin, and H.-J. Jacobasch, J. Colloid Interface Sci. 208, 329 (1998).

    Article  CAS  Google Scholar 

  20. R. Zimmermann, T. Osaki, R. Schweiss, and C. Werner, Microfluid. Nanofluid. 2, 367 (2006).

    Article  CAS  Google Scholar 

  21. R. Schweiss, P. Welzel, W. Knoll, and C. Werner, Chem. Commun. (Cambridge) 2005, p. 256.

  22. R. Zimmermann, O. Birkert, G. Gauglitz, and C. Werner, ChemPhysChem 4, 509 (2003).

    Article  CAS  Google Scholar 

  23. R. Zimmermann, W. Norde, M. A. Cohen Stuart, and C. Werner, Langmuir 21, 5108 (2005).

    Article  CAS  Google Scholar 

  24. A. Härtl, S. Nowy, R. Zimmermann, C. Werner, D. Horinek, R. Netz, and M. Stutzmann, J. Am. Chem. Soc. 129, 1287 (2007).

    Article  Google Scholar 

  25. G. Gauglitz, J. Krause-Bonte, H. Schlemmer, and A. Matthes, Anal. Chem. 60, 2609 (1988).

    Article  CAS  Google Scholar 

  26. D. Beyerlein, G. Belge, K.-J. Eichhorn, G. Gauglitz, K. Grundke, and B. Voit, Macromol. Symp. 164, 117 (2001).

    Article  CAS  Google Scholar 

  27. G. Gauglitz, A. Brecht, G. Kraus, and W. Nahm, Sens. Actuators B 11, 21 (1993).

    Article  Google Scholar 

  28. J. Piehler, A. Brecht, G. Gauglitz, C. Maul, M. Zerlin, R. Thiericke, and S. Grabley, Anal. Biochem. 249, 94 (1997).

    Article  CAS  Google Scholar 

  29. O. Birkert, H.-M. Haake, A. Schütz, J. Mack, A. Brecht, G. Jung, and G. Gauglitz, Anal. Biochem. 282, 200 (2000).

    Article  CAS  Google Scholar 

  30. H.-M. Schmitt, A. Brecht, J. Piehler, and G. Gauglitz, Biosens. Bioelectron. 12, 809 (1997).

    Article  CAS  Google Scholar 

  31. O. Birkert and G. Gauglitz, Anal. Bioanal. Chem. 372, 141 (2002).

    Article  CAS  Google Scholar 

  32. B. P. Möhrle, K. Köhler, J. Jaehrling, R. Brock, and G. Gauglitz, Anal. Bioanal. Chem. 384, 407 (2005).

    Article  Google Scholar 

  33. R. Zimmermann, C. Werner, K. J. Eichhorn, and G. Gauglitz, Patent DE 102 05 775, IPF, Universität Tübingen (7 February 2002).

  34. R. Zimmermann, T. Osaki, T. Kratzmüller, G. Gauglitz, S. S. Dukhin, and C. Werner, Anal. Chem. 78, 5851 (2006).

    Article  CAS  Google Scholar 

  35. S. S. Dukhin, R. Zimmermann, and C. Werner, J. Colloid Interface Sci. 274, 309 (2004).

    Article  CAS  Google Scholar 

  36. J. F. L. Duval and H. P. van Leeuwen, Langmuir 20, 10324 (2004).

  37. C. Hänel and G. Gauglitz, Anal. Bioanal. Chem. 372, 91 (2002).

    Article  Google Scholar 

  38. G. Gauglitz, Anal. Bioanal. Chem. 381, 141 (2005).

    Article  CAS  Google Scholar 

  39. T. Osaki and C. Werner, Langmuir 19, 5787 (2003).

    Article  CAS  Google Scholar 

  40. T. Pompe, S. Zschoche, K. Salchert, N. Herold, M. F. Gouzy, C. Sperling, and C. Werner, Biomacromolecules 4, 1072 (2003).

    Article  CAS  Google Scholar 

  41. J. A. de Feijter, J. Benjamins, and F. A. Veer, Biopolymers 17, 1759 (1978).

    Article  Google Scholar 

  42. J. Vörös, Biophys. J. 87, 553 (2004).

    Article  Google Scholar 

  43. E. G. Young, in Comprehensive Biochemistry, Vol. 7: Proteins (Part 1), edited by M. Florkin and E. H. Stotz (Elsevier, Amsterdam, 1963), pp. 1–55.

    Google Scholar 

  44. P. G. Righetti and T. Caravaggio, J. Chromatogr. 127, 1 (1976).

    Article  CAS  Google Scholar 

  45. W. Norde, in Biopolymers at Interfaces, edited by M. Malmsten (Marcel Dekker, New York, 1998), pp. 27–54.

    Google Scholar 

  46. A. Nadarajah, C. F. Lu, and K. K. Chittur, in Proteins at Interfaces II: Fundamentals and Applications, edited by T. A. Horbett and J. L. Brash (American Chemical Society, Washington, DC, 1995), pp. 181–194.

    Chapter  Google Scholar 

  47. C. A. Haynes and W. Norde, Colloids Surf., B 2, 517 (1994).

    Article  CAS  Google Scholar 

  48. W. Norde, Macromol. Symp. 103, 5 (1996).

    Article  CAS  Google Scholar 

  49. W. Norde and C. A. Haynes, in Proteins at Interfaces II: Fundamentals and Applications, edited by T. A. Horbett and J. L. Brash (American Chemical Society, Washington, DC, 1995), pp. 26–40.

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zimmermanna, R., Osaki, T., Gauglitz, G. et al. Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes. Biointerphases 2, 159–164 (2007). https://doi.org/10.1116/1.2814066

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1116/1.2814066