Biointerphases

Journal for Biophysical Chemistry

Biointerphases Cover Image
Open Access

Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes

  • Ralf Zimmermanna1,
  • Toshihisa Osaki1,
  • Günter Gauglitz2 and
  • Carsten Werner1, 3
Biointerphases2:20400159

https://doi.org/10.1116/1.2814066

Received: 1 October 2007

Accepted: 29 October 2007

Abstract

Streaming potential/current measurements for the characterization of charge formation processes at solid/liquid interfaces were combined with reflectometric interference spectroscopy. The simultaneous determination of electrosurface characteristics and the optical thickness of interfacial layers provides information on structural variations of adsorbed or covalently bound polymers and on charge dependent adsorption and desorption phenomena at solid/liquid interfaces. To demonstrate the potentialities of this extended approach for biointerfacial studies the authors report a series of experiments on the adsorption of the plasma protein fibrinogen at poly(octadecene-alt-maleic acid) thin films.