Journal for Biophysical Chemistry

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Open Access

Surface supported bilayer platform for studies of lateral association of proteins in membranes (Mini Review)

  • Mikhail Merzlyakov1,
  • Edwin Li1 and
  • Kalina Hristova1

Received: 26 November 2007

Accepted: 6 June 2008


Here, the authors review how surface supported bilayers can be engineered and how Förster resonance energy transfer (FRET) can be used to quantify interactions between transmembrane peptides in these bilayers. The requirements for the surface supported platform are (1) lateral mobility of the peptides, (2) transmembrane orientation of the peptides, and (3) capabilities for FRET measurements. To satisfy these requirements, a new assembly method, termed “directed assembly” was developed. This assembly method could have broad utility in basic studies of proteins in membranes and in biotechnological applications.