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Biointerphases

Journal for Biophysical Chemistry

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Black lipid membranes stabilized through substrate conjugation to a hydrogel

Biointerphases volume 3, pages FA96–FA100 (2008)Cite this article

Abstract

Recent research in stabilizing lipid bilayer membranes has been directed toward tethering the membrane to a solid surface or contacting the membrane with a solid support such as a gel. It is also known that the solvent annulus plays an important role in lipid bilayer stability. In this work, the authors set out to stabilize the solvent annulus. Glass substrates with ∼500 μm apertures were functionalized with 3-methacryloxypropyltrimethoxysilane to allow cross-linking with a surrounding polyethyleneglycol dimethacrylate hydrogel. The hydrogel makes a conformal mold around both the lipid bilayer and the solvent reservoir. Since the hydrogel is covalently conjugated with the glass substrate via vinyl groups, the solvent annulus is prevented from leaving the aperture boundary. Measurements of a membrane created with this approach showed that it remained a stable bilayer with a resistance greater than 1 GΩ( for 12 days. Measurements of the ion channel gramicidin A, α-hemolysin, and alamethicin incorporated into these membranes showed the same conductance behavior as conventional membranes.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, University of California, 90095, Los Angeles, California

    Tae-Joon Jeon

  2. Department of Bioengineering, University of California, 90095, Los Angeles, California

    Tae-Joon Jeon & Jason L. Poulos

  3. Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 90089, Los Angeles, California

    Noah Malmstadt

  4. Department of Bioengineering and California Nanosystems Institute, University of California, 90095, Los Angeles, California

    Jacob J. Schmidt

Authors
  1. Tae-Joon Jeon
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  2. Noah Malmstadt
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  3. Jason L. Poulos
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  4. Jacob J. Schmidt
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Correspondence to Jacob J. Schmidt.

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Jeon, TJ., Malmstadt, N., Poulos, J.L. et al. Black lipid membranes stabilized through substrate conjugation to a hydrogel. Biointerphases 3, FA96–FA100 (2008). https://doi.org/10.1116/1.2948314

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  • DOI: https://doi.org/10.1116/1.2948314