Skip to main content

Journal for Biophysical Chemistry

Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold

Abstract

The conformational changes in elastinlike polypeptides (ELPs) grafted to a solid/solution interface via different architectures were studied using surface plasmon resonance spectroscopy and surface plasmon field-enhanced fluorescence spectroscopy (SPFS). SPFS provides a simple and convenient optical method to study the influence of the grafting method and the graft density on the conformational changes in ELPs at the solid-solution interface as a function of environmental variables. A typical response of the ELP, consistent with its stimuli responsiveness, was a gradual collapse upon increasing the ionic strength; this effect was inversely correlated with the surface graft density of the ELP.

References

  1. D. E. Meyer and A. Chilkoti, Biomacromolecules 3, 357 (2002).

    Article  CAS  Google Scholar 

  2. K. Trabbic-Carlson, L. A. Setton, and A. Chilkoti, Biomacromolecules 4, 572 (2003).

    Article  CAS  Google Scholar 

  3. J. A. Hubbell, Curr. Opin. Biotechnol. 10, 123 (1999).

    Article  CAS  Google Scholar 

  4. S. E. Sakiyama-Elbert and J. A. Hubbell, Annu. Rev. Mater. Res. 31, 183 (2001).

    Article  CAS  Google Scholar 

  5. R. Langer, Nature (London) 392, 5 (1998).

    CAS  Google Scholar 

  6. H. Kanazawa, Y. Matsushima, and T. Okano, Adv. Chromatogr. 41, 311 (2001).

    CAS  Google Scholar 

  7. P. S. Stayton, T. Shimoboji, C. Long, A. Chilkoti, G. Chen, J. M. Harris, and A. S. Hoffman, Nature (London) 378, 472 (1995).

    Article  CAS  Google Scholar 

  8. H. Betre, L. A. Setton, D. E. Meyer, and A. Chilkoti, Biomacromolecules 3, 910 (2002).

    Article  CAS  Google Scholar 

  9. S. Fujishige, K. Kubota, and I. Ando, J. Phys. Chem. 93, 3311 (1989).

    Article  CAS  Google Scholar 

  10. D. E. Meyer and A. Chilkoti, Biomacromolecules 5, 846 (2004).

    Article  CAS  Google Scholar 

  11. M. Miao, C. M. Bellingham, R. J. Stahl, E. E. Sitarz, C. J. Lane, and F. W. Keeley, J. Biol. Chem. 278, 48553 (2003).

    Article  CAS  Google Scholar 

  12. K. Kontturi, S. Mafe, J. A. Manyanares, B. L. Svarfvar, and P. Viinikka, Macromolecules 29, 5740 (1996).

    Article  CAS  Google Scholar 

  13. R. A. Siegel and B. A. Firestone, Macromolecules 21, 3254 (1988).

    Article  CAS  Google Scholar 

  14. I. C. Kwon, Y. H. Bae, and S. W. Kim, Nature (London) 354, 291 (1991).

    Article  CAS  Google Scholar 

  15. D. Kuckling, I. G. Ivanova, H. J. P. Adler, and T. Wolff, Polymer 43, 1813 (2002).

    Article  CAS  Google Scholar 

  16. J. H. Holtz and S. A. Asher, Nature (London) 389, 829 (1997).

    Article  CAS  Google Scholar 

  17. T. Miyata, N. Asami, and T. Uragami, Nature (London) 399, 766 (1999).

    Article  CAS  Google Scholar 

  18. N. Nath and A. Chilkoti, J. Am. Chem. Soc. 123, 8197 (2001).

    Article  CAS  Google Scholar 

  19. J. Hyun, W. K. Lee, N. Nath, A. Chilkoti, and S. Zauscher, J. Am. Chem. Soc. 126, 7330 (2004).

    Article  CAS  Google Scholar 

  20. D. C. Chow, M. R. Dreher, K. Trabbic-Carlson, and A. Chilkoti, Biotechnol. Prog. 22, 638 (2006).

    Article  CAS  Google Scholar 

  21. D. E. Meyer and A. Chilkoti, Nat. Biotechnol. 17, 1112 (1999).

    Article  CAS  Google Scholar 

  22. See http://probes.invitrogen.com, Chap. 1; http://probes.invitrogen.com/handbook/sections/0100.html, the handbook.

  23. D. Y. Furgeson, M. R. Dreher, and A. Chilkoti, J. Controlled Release 110, 362 (2006).

    Article  CAS  Google Scholar 

  24. S. Löfås and B. J. Johnsson, J. Chem. Soc., Chem. Commun. 1990, 1526.

  25. W. Frey, D. E. Meyer, and A. Chilkoti, Langmuir 19, 1641 (2003).

    Article  CAS  Google Scholar 

  26. T. Liebermann and W. Knoll, Colloids Surf., A 171, 115 (2000).

    Google Scholar 

  27. R. G. Chapman, E. Ostuni, L. Yan, and G. M. Whitesides, Langmuir 16, 6927 (2000).

    Article  CAS  Google Scholar 

  28. P. B. Garland, Q. Rev. Biophys. 29, 91 (1996).

    Article  CAS  Google Scholar 

  29. W. Knoll, H. Park, E. Sinner, D. Yao, and F. Yu, Surf. Sci. 570, 30 (2004).

    Article  CAS  Google Scholar 

  30. K. Vasilev, W. Knoll, and M. Kreitera, J. Chem. Phys. 120, 3439 (2004).

    Article  CAS  Google Scholar 

  31. E. Reimhult, C. Larsson, B. Kasemo, and F. Hook, Anal. Chem. 76, 7211 (2004).

    Article  CAS  Google Scholar 

  32. F. Yu, B. Persson, S. Lofas, and W. Knoll, J. Am. Chem. Soc. 126, 8902 (2004).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, F., Joon, H.M., Trabbic-Carlson, K. et al. Surface plasmon optical study of the interfacial phase transition of elastinlike polypeptide grafted on gold. Biointerphases 3, 66–74 (2008). https://doi.org/10.1116/1.2965133

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1116/1.2965133