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Biointerphases

Journal for Biophysical Chemistry

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Neutron reflectometry to investigate the delivery of lipids and DNA to interfaces (Review)

Biointerphases volume 3, pages FB64–FB82 (2008)Cite this article

Abstract

The application of scattering methods in the study of biological and biomedical problems is a field of research that is currently experiencing fast growth. In particular, neutron reflectometry (NR) is a technique that is becoming progressively more widespread, as indicated by the current commissioning of several new reflectometers worldwide. NR is valuable for the characterization of biomolecules at interfaces due to its capability to provide quantitative structural and compositional information on relevant molecular length scales. Recent years have seen an increasing number of applications of NR to problems related to drug and gene delivery. We start our review by summarizing the experimental methodology of the technique with reference to the description of biological liquid interfaces. Various methods for the interpretation of data are then discussed, including a new approach based on the lattice mean-field theory to help characterize stimulus-responsive surfaces relevant to drug delivery function. Recent progress in the subject area is reviewed in terms of NR studies relevant to the delivery of lipids and DNA to surfaces. Lastly, we discuss two case studies to exemplify practical features of NR that are exploited in combination with complementary techniques. The first case concerns the interactions of lipid-based cubic phase nanoparticles with model membranes a drug delivery application, and the second case concerns DNA compaction at surfaces and in the bulk solution a gene delivery application.

References

  1. J. Penfold and R. K. Thomas, J. Phys.: Condens. Matter 2, 1369 (1990).

    Article  CAS  Google Scholar 

  2. J. Penfold, Curr. Opin. Colloid Interface Sci. 7, 139 (2002).

    Article  CAS  Google Scholar 

  3. J. E. Bradley, E. M. Lee, R. K. Thomas, A. J. Willatt, J. Penfold, R. C. Ward, D. P. Gregory, and W. Waschkowski, Langmuir 4, 821 (1988).

    Article  CAS  Google Scholar 

  4. T. L. Crowley, E. M. Lee, E. A. Simister, and R. K. Thomas, Physica B Amsterdam 173, 143 (1991).

    Article  Google Scholar 

  5. J. R. Lu, T. J. Su, R. K. Thomas, J. Penfold, and R. W. Richards, Polymer 37, 109 (1996).

    Article  CAS  Google Scholar 

  6. H. Xu, S. Perumal, X. Zhao, N. Du, X.-Y. Liu, Z. Jia, and J. R. Lu, Biophys. J. 94, 4405 (2008).

    Article  CAS  Google Scholar 

  7. V. F. Sears, Neutron News 3, 29 (1992).

    Article  Google Scholar 

  8. C. F. Majkrzak, S. K. Satija, N. F. Berk, S. K. Krueger, J. A. Borchers, J. A. Dura, R. Ivkov, and K. ODonovan, Neutron News 12, 25 (2001).

    Article  Google Scholar 

  9. R. Cubitt and G. Fragneto, Appl. Phys. A: Mater. Sci. Process. 74, S329 (2002).

    Article  CAS  Google Scholar 

  10. J. Lekner, Theory of Reflection of Electromagnetic and Particle Waves (Martinus Nijhoff, Dordrecht, 1987).

    Google Scholar 

  11. L. G. Parratt, Phys. Rev. 95, 359 (1954).

    Article  Google Scholar 

  12. F. Abelès, Ann. Phys. (Paris) 5, 596 (1950).

    Google Scholar 

  13. M. Born and E. Wolf, Prinicples of Optics, 6th ed. (Cambridge University Press Cambridge, England, 1980).

    Google Scholar 

  14. D. J. Lyttle, J. R. Lu, T. J. Su, R. K. Thomas, and J. Penfold, Langmuir 11, 1001 (1995).

    Article  CAS  Google Scholar 

  15. J. R. Lu, R. K. Thomas, and J. Penfold, Adv. Colloid Interface Sci. 84, 143 (2000).

    Article  CAS  Google Scholar 

  16. G. Fragneto, J. R. Lu, D. C. McDermott, R. K. Thomas, A. R. Rennie, P. D. Gallagher, and S. K. Satija, Langmuir 12, 477 (1996).

    Article  CAS  Google Scholar 

  17. S. Krueger, J. F. Ankner, S. K. Satija, C. F. Majkrzak, D. Gurley, and M. Colombini, Langmuir 11, 3218 (1995).

    Article  CAS  Google Scholar 

  18. Neutron Scattering in Biology, edited by J. Fitter, T. Gutberlet, and J. Katsaras (Springer, Berlin, 2006).

    Google Scholar 

  19. R. K. Thomas, Annu. Rev. Phys. Chem. 55, 391 (2004).

    Article  CAS  Google Scholar 

  20. T. Nylander, F. Tiberg, T. S. Su, J. R. Lu, and R. K. Thomas, Biomacromolecules 2, 278 (2001).

    Article  CAS  Google Scholar 

  21. J. R. Lu, Annu. Rep. Prog. Chem., Sect. C: Phys. Chem. 95, 3 (1999).

    Article  CAS  Google Scholar 

  22. M. Cárdenas, T. Arnebrant, A. Rennie, G. Fragneto, R. K. Thomas, and L. Lindh, Biomacromolecules 8, 65 (2007).

    Article  CAS  Google Scholar 

  23. M. Cárdenas and T. Nylander, in Interaction of DNA with Surfactants and Polymers, edited by B. Lindman and R. Dias (Wiley, Oxford, 2008), pp. 291–316.

    Chapter  Google Scholar 

  24. M. Cárdenas, C. A. Dreiss, T. Nylander, C. P. Chan, T. Cosgrove, and B. Lindman, Langmuir 21, 3578 (2005).

    Article  CAS  Google Scholar 

  25. P. Vandoolaeghe, A. R. Rennie, R. A. Campbell, R. K. Thomas, F. Höök, G. Fragneto, F. Tiberg, and T. Nylander, Soft Matter 4, 2267 (2008).

    Article  CAS  Google Scholar 

  26. H. P. Vacklin, F. Tiberg, and R. K. Thomas, Biochim. Biophys. Acta 1668, 17 (2005).

    Article  CAS  Google Scholar 

  27. H. P. Vacklin, F. Tiberg, G. Fragneto, and R. K. Thomas, Langmuir 21, 2827 (2005).

    Article  CAS  Google Scholar 

  28. A. Yaseen, J. R. Lu, J. R. P. Webster, and J. Penfold, Biophys. Chem. 117, 263 (2005).

    Article  CAS  Google Scholar 

  29. A. P. Le Brun, S. A. Holt, D. S. Shah, C. F. Majkrzak, and J. H. Lakey, Eur. Biophys. J. 37, 639 (2008).

    Article  CAS  Google Scholar 

  30. L. Nevot and P. Croce, Rev. Phys. Appl. 15, 761 (1980).

    Article  CAS  Google Scholar 

  31. See http://www.hmi.de/bensc/instrumentation/instrumente/v6/refl/parratt_en.htm for a description of the computer programs and access to the software.

  32. See http://material.fysik.uu.se/Group_members/adrian/reflect.htm Analysis for a description of the computer programs and access to the software.

  33. A. Nelson, J. Appl. Crystallogr. 39, 273 (2006).

    Article  CAS  Google Scholar 

  34. M. Björck and G. Andersson, J. Appl. Crystallogr. 40, 1174 (2007).

    Article  CAS  Google Scholar 

  35. See http://material.fysik.uu.se/Group_members/adrian/cprof.htm for an account of the specific computer programs.

  36. See http://material.fysik.uu.se/Group_members/adrian/refprog.htm for an account of the specific computer programs.

  37. J. Penfold, D. S. Sivia, E. Staples, I. Tucker, and R. K. Thomas, Langmuir 20, 2265 (2004).

    Article  CAS  Google Scholar 

  38. T. P. Russell, Physica B Amsterdam 221, 267 (1996).

    Article  CAS  Google Scholar 

  39. J. Zhang, T. Nylander, R. A. Campbell, A. R. Rennie, S. Zauscher, and P. Linse, Soft Matter 4, 500 (2008).

    Article  CAS  Google Scholar 

  40. J. M. H. M. Scheutjens and G. J. Fleer, J. Phys. Chem. 83, 1619 (1979).

    Article  CAS  Google Scholar 

  41. G. J. Fleer, M. A. Cohen Stuart, J. M. H. M. Scheutjens, T. Cosgrove, and B. Vincent, Polymers at Interfaces (Chapman & Hall, London, 1993).

    Google Scholar 

  42. P. J. Flory, Principles of Polymer Chemistry (Cornell University Press, Ithaca, NY, 1953).

    Google Scholar 

  43. G. Karlström, J. Phys. Chem. 89, 4962 (1985).

    Article  Google Scholar 

  44. P. Linse and M. Björling, Macromolecules 24, 6700 (1991).

    Article  CAS  Google Scholar 

  45. P. Linse and T. A. Hatton, Langmuir 13, 4066 (1997).

    Article  CAS  Google Scholar 

  46. J. E. Dennis, Jr. and R. B. Schnabel, Numerical Methods for Unconstrained Optimization and Nonlinear Equations (Prentice-Hall, Englewood Cliffs, 1983).

    Google Scholar 

  47. M. Landgren and B. Jonsson, J. Phys. Chem. 97, 1656 (1993).

    Article  CAS  Google Scholar 

  48. R. M. Azzam and N. M. Bashara, Ellipsometry and Polarized Light (North-Holland, Amsterdam, 1977).

    Google Scholar 

  49. J. A. Defeijter, J. Benjamins, and F. A. Veer, Biopolymers 17, 1759 (1978).

    Article  CAS  Google Scholar 

  50. M. Rodahl, F. Hook, C. Fredriksson, C. A. Keller, A. Krozer, P. Brzezinski, M. Voinova, and B. Kasemo, Faraday Discuss. 107, 229 (1997).

    Article  CAS  Google Scholar 

  51. F. Höök, B. Kasemo, T. Nylander, C. Fant, K. Sott, and E. H. Elwing, Anal. Chem. 73, 5796 (2001).

    Article  CAS  Google Scholar 

  52. J. S. Pedersen, Adv. Colloid Interface Sci. 70, 171 (1997).

    Article  CAS  Google Scholar 

  53. A. D. Bangham and R. W. Horne, J. Mol. Biol. 8, 660 (1964).

    Article  CAS  Google Scholar 

  54. G. Gregoriadis, C. P. Swain, E. J. Wills, and A. S. Tavill, Lancet 303, 1313 (1974).

    Article  Google Scholar 

  55. P. Couvreur and C. Vauthier, Pharm. Res. 23, 1417 (2006).

    Article  CAS  Google Scholar 

  56. S. Ganta, H. Devalapally, A. Shahiwala, and M. Amiji, J. Controlled Release 126, 187 (2008).

    Article  CAS  Google Scholar 

  57. M. C. Woodle and D. D. Lasic, Biochim. Biophys. Acta 1113, 171 (1992).

    CAS  Google Scholar 

  58. D. Papahadjopoulos et al., Proc. Natl. Acad. Sci. U.S.A. 88, 11460 (1991).

    Article  CAS  Google Scholar 

  59. S. I. Jeon, J. H. Lee, J. D. Andrade, and P. G. De Gennes, J. Colloid Interface Sci. 142, 149 (1991).

    Article  CAS  Google Scholar 

  60. L. M. Ickenstein, M. C. Arfvidsson, D. Needham, L. D. Mayera, and K. Edwards, Biochim. Biophys. Acta 1614, 135 (2003).

    Article  CAS  Google Scholar 

  61. J. S. Patton and M. C. Carey, Science 204, 145 (1979).

    Article  CAS  Google Scholar 

  62. K. Larsson, J. Phys. Chem. 93, 7304 (1989).

    Article  CAS  Google Scholar 

  63. W. Buchheim and K. Larsson, J. Colloid Interface Sci. 117, 582 (1987).

    Article  CAS  Google Scholar 

  64. M. Monduzzi, H. Ljusberg-Wahren, and K. Larsson, Langmuir 16, 7355 (2000).

    Article  CAS  Google Scholar 

  65. K. Larsson, Curr. Opin. Colloid Interface Sci. 5, 64 (2000).

    Article  CAS  Google Scholar 

  66. T. Landh, J. Phys. Chem. 98, 8453 (1994).

    Article  CAS  Google Scholar 

  67. J. Gustafsson, H. Ljusberg-Wahren, M. Almgren, and K. Larsson, Langmuir 12, 4611 (1996).

    Article  CAS  Google Scholar 

  68. J. Barauskas, M. Johnsson, F. Johnson, and F. Tiberg, Langmuir 21, 2569 (2005).

    Article  CAS  Google Scholar 

  69. J. Barauskas, M. Johnsson, and F. Tiberg, Nano Lett. 5, 1615 (2005).

    Article  CAS  Google Scholar 

  70. Delivery and Controlled Release of Bioactives in Foods and Nutraceuticals, edited by N. Garti (Woodhead, Cambridge, 2008).

    Google Scholar 

  71. E. Acosta, in Delivery and Controlled Release of Bioactives in Foods and Nutraceuticals, edited by N. Garti (Woodhead, Cambridge, 2008), pp.53–106.

    Chapter  Google Scholar 

  72. T. Niidome and L. Huang, Gene Ther. 9, 1647 (2002).

    Article  CAS  Google Scholar 

  73. K. K. Ewert, C. E. Samuel, and C. R. Safinya, in DNA Interactions with Polymers and Surfactants, edited by R. Dias and B. Lindman (Wiley, Hoboken, NJ, 2008), pp. 377–404.

    Chapter  Google Scholar 

  74. E. Sackmann, Science 271, 43 (1996).

    Article  CAS  Google Scholar 

  75. R. P. Richter, R. Berat, and A. R. Brisson, Langmuir 22, 3497 (2006).

    Article  CAS  Google Scholar 

  76. F. Tiberg, I. Harwigsson, and M. Malmsten, Eur. Biophys. J. 29, 196 (2000).

    Article  CAS  Google Scholar 

  77. T. Gutberlet, R. Steitz, G. Fragneto, and B. Klösgen, J. Phys.: Condens. Matter 16, S2469 (2004).

    Article  CAS  Google Scholar 

  78. D. Stroumpoulis, A. Parra, and M. Tirrell, AIChE J. 52, 2931 (2006).

    Article  CAS  Google Scholar 

  79. C. A. Keller and B. Kasemo, Biophys. J. 75, 1397 (1998).

    Article  CAS  Google Scholar 

  80. H. P. Wacklin and R. K. Thomas, Langmuir 23, 7644 (2007).

    Article  CAS  Google Scholar 

  81. L. M. Grant and F. Tiberg, Biophys. J. 82, 1373 (2002).

    Article  CAS  Google Scholar 

  82. J. Y. Wong, J. Majewski, M. Seitz, C. K. Park, J. N. Israelachvili, and G. S. Smith, Biophys. J. 77, 1445 (1999).

    Article  CAS  Google Scholar 

  83. U. A. Perez-Salas, K. M. Faucher, C. F. Majkrzak, N. F. Berk, S. Krueger, and E. L. Chaikof, Langmuir 19, 7688 (2003).

    Article  CAS  Google Scholar 

  84. M. Tanaka and E. Sackmann, Nature London 437, 656 (2005).

    Article  CAS  Google Scholar 

  85. G. Valincius, D. J. McGillivray, W. Febo-Ayala, D. J. Vanderah, J. J. Kasianowicz, and M. Lösche, J. Phys. Chem. B 110, 10213 (2006).

    Article  CAS  Google Scholar 

  86. D. J. McGillivray, G. Valincius, D. J. Vanderah, W. Febo-Ayala, J. T. Woodward, F. Heinrich, J. J. Kasianowicz, and M. Lösche, BioInterphases 2, 21 (2007).

    Article  CAS  Google Scholar 

  87. B. A. Cornell, V. L. B. Braach-Maksvytis, L. G. King, P. D. J. Osman, B. Raguse, L. Wieczorek, and R. J. Pace, Nature London 387, 580 (1997).

    Article  CAS  Google Scholar 

  88. D. A. Doshi, A. M. Dattelbaum, E. B. Watkins, C. J. Brinker, B. I. Swanson, A. P. Shreve, A. N. Parikh, and J. Majewski, Langmuir 21, 2865 (2005).

    Article  CAS  Google Scholar 

  89. A. V. Hughes, J. R. Howse, A. Dabkowska, R. A. L. Jones, M. J. Lawrence, and S. J. Roser, Langmuir 24, 1989 (2008).

    Article  CAS  Google Scholar 

  90. H. Haas, R. Steitz, A. Fasano, G. M. Liuzzi, E. Polverini, P. Cavatorta, and P. Riccio, Langmuir 23, 8491 (2007).

    Article  CAS  Google Scholar 

  91. G. Fragneto and M. Rheinstädter, C. R. Phys. 8, 865 (2007).

    Article  CAS  Google Scholar 

  92. C. Li, D. Constantin, and T. Salditt, J. Phys.: Condens. Matter 16, S2439 (2004).

    Article  CAS  Google Scholar 

  93. N. Kucerka, M.-P. Nieh, J. Pencer, T. Harroun, and J. Katsaras, Curr. Opin. Colloid Interface Sci. 12, 17 (2007).

    Article  CAS  Google Scholar 

  94. P. Callow, G. Fragneto, R. Cubitt, D. J. Barlow, M. J. Lawrence, and P. Timmins, Langmuir 21, 7912 (2005).

    Article  CAS  Google Scholar 

  95. G. Wagner, A. Bancaud, J.-P. Quivy, C. Clapier, G. Almouzni, and J.-L. Viovy, Biophys. J. 89, 3647 (2005).

    Article  CAS  Google Scholar 

  96. K. Matsubara, N. Sano, T. Umehara, and M. Horikoshi, Genes Cells 12, 13 (2007).

    Article  CAS  Google Scholar 

  97. A. Bertin, A. Leforestier, D. Durand, and F. Livolant, Biochemistry 43, 4773 (2004).

    Article  CAS  Google Scholar 

  98. S. A. Gani, D. C. Mukherjee, and D. K. Chattoraj, Langmuir 15, 7130 (1999).

    Article  CAS  Google Scholar 

  99. A. Elaissari, P. Cros, C. Pichot, V. Laurent, and B. Mandrand, Colloids Surf., A 83, 25 (1994).

    Article  CAS  Google Scholar 

  100. V. Balladur, A. Theretz, and B. Mandrand, J. Colloid Interface Sci. 194, 408 (1997).

    Article  CAS  Google Scholar 

  101. R. R. Kunze and R. R. Netz, Phys. Rev. Lett. 85, 4389 (2000).

    Article  CAS  Google Scholar 

  102. I. M. Verma and N. Somia, Nature London 389, 239 (1997).

    Article  CAS  Google Scholar 

  103. N. S. Templeton and D. D. Lasic, Mol. Biotechnol. 11, 175 (1999).

    Article  CAS  Google Scholar 

  104. D. K. Chattoraj, P. Chowrashi, and K. Chakravarti, Biopolymers 5, 173 (1967).

    Article  CAS  Google Scholar 

  105. K. Eskilsson, C. Leal, B. Lindman, M. Miguel, and T. Nylander, Langmuir 17, 1666 (2001).

    Article  CAS  Google Scholar 

  106. M. Cárdenas, K. Schillen, T. Nylander, J. Jansson, and B. Lindman, Phys. Chem. Chem. Phys. 6, 1603 (2004).

    Article  CAS  Google Scholar 

  107. M. Cárdenas, A. Braem, T. Nylander, and B. Lindman, Langmuir 19, 7712 (2003).

    Article  CAS  Google Scholar 

  108. L. Stryer, Biochemistry (Freeman, New York, 1995).

    Google Scholar 

  109. A. Elaissari, Y. Chevalier, F. Ganachaud, T. Delair, and C. Pichot, Langmuir 16, 1261 (2000).

    Article  CAS  Google Scholar 

  110. D. Zanchet, C. M. Michel, W. J. Parak, D. Gerion, and A. P. Alivisatos, Nano Lett. 1, 32 (2001).

    Article  CAS  Google Scholar 

  111. S. J. Park, A. A. Lazarides, J. J. Storhoff, L. Pesce, and C. A. Mirkin, J. Phys. Chem. B 108, 12375 (2004).

    Article  CAS  Google Scholar 

  112. L. Olofsson, T. Rindzevicius, I. Pfeiffer, M. Käll, and F. Höök, Langmuir 19, 10414 (2003).

    Article  CAS  Google Scholar 

  113. S. Moses et al., Langmuir 20, 11134 (2004).

    Article  CAS  Google Scholar 

  114. R. Levicky, T. M. Herne, M. J. Tarlov, and S. K. Satija, J. Am. Chem. Soc. 120, 9787 (1998).

    Article  CAS  Google Scholar 

  115. C.-Y. Lee, P. Gong, G. M. Harbers, D. W. Grainger, D. G. Castner, and L. J. Gamble, Anal. Chem. 78, 3316 (2006).

    Article  CAS  Google Scholar 

  116. P. Gong, C.-Y. Lee, L. J. Gamble, D. G. Castner, and D. W. Grainger, Anal. Chem. 78, 3326 (2006).

    Article  CAS  Google Scholar 

  117. L. M. Demers, C. A. Mirkin, R. C. Mucic, R. A. Reynolds, R. L. Letsinger, R. Elghanian, and G. Viswanadham, Anal. Chem. 72, 5535 (2000).

    Article  CAS  Google Scholar 

  118. S. M. Melnikov, V. G. Sergeyev, and K. Yoshikawa, J. Am. Chem. Soc. 117, 9951 (1995).

    Article  CAS  Google Scholar 

  119. R. Dias, S. Melnikov, B. Lindman, and M. G. Miguel, Langmuir 16, 9577 (2000).

    Article  CAS  Google Scholar 

  120. M. Cárdenas, T. Nylander, R. K. Thomas, and B. Lindman, Langmuir 21, 6495 (2005).

    Article  CAS  Google Scholar 

  121. J. Zhang, D. J. F. Taylor, P. X. Li, R. K. Thomas, J. B. Wang, and J. Penfold, Langmuir 24, 1863 (2008).

    Article  CAS  Google Scholar 

  122. J.-C. Wu, T.-L. Lin, U.-S. Jeng, and N. Torikai, Physica B Amsterdam 385–386, 838 (2006).

    Article  CAS  Google Scholar 

  123. J.-C. Wu, T.-L. Lin, U.-S. Jeng, H.-Y. Lee, and T. Gutberlet, Physica B Amsterdam 385–386, 841 (2006).

    Article  CAS  Google Scholar 

  124. X. Chen, J. Wang, N. Shen, Y. Luo, L. Li, M. Liu, and R. K. Thomas, Langmuir 18, 6222 (2002).

    Article  CAS  Google Scholar 

  125. J. Generosi, C. Castellano, D. Pozzi, A. C. Castellanon, R. Felici, F. Natali, and G. Fragneto, J. Appl. Phys. 96, 6839 (2004).

    Article  CAS  Google Scholar 

  126. P. T. Spicer, Curr. Opin. Colloid Interface Sci. 10, 274 (2005).

    Article  CAS  Google Scholar 

  127. L. Sagalowicz, R. Mezzenga, and M. E. Leser, Curr. Opin. Colloid Interface Sci. 11, 224 (2006).

    Article  CAS  Google Scholar 

  128. P. Vandoolaeghe, F. Tiberg, and T. Nylander, Langmuir 22, 9169 (2006).

    Article  CAS  Google Scholar 

  129. M. Cárdenas, J. Campos-Teran, T. Nylander, and B. Lindman, Langmuir 20, 8597 (2004).

    Article  CAS  Google Scholar 

  130. R. Bruinsma, Eur. Phys. J. B 4, 75 (1998).

    Article  CAS  Google Scholar 

  131. R. Chatterjee and D. K. Chattoraj, Biopolymers 18, 147 (1979).

    Article  CAS  Google Scholar 

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  1. Physical Chemistry 1, Lund University, Box 124, SE-221 00, Lund, Sweden

    Tommy Nylander, Pauline Vandoolaeghe & Per Linse

  2. Institut Laue-Langevin, BP 156, 38042, Grenoble Cedex 9, France

    Richard A. Campbell

  3. Biomedical Technology Laboratory, Faculty of Health and Society, Malmö University, SE-205 06, Malmö, Sweden

    Marité Cárdenas

  4. Department of Physics, Uppsala University, Box 530, SE-751 21, Uppsala, Sweden

    Adrian R. Rennie

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Nylander, T., Campbell, R.A., Vandoolaeghe, P. et al. Neutron reflectometry to investigate the delivery of lipids and DNA to interfaces (Review). Biointerphases 3, FB64–FB82 (2008). https://doi.org/10.1116/1.2976448

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