Biointerphases

Journal for Biophysical Chemistry

Biointerphases Cover Image
Open Access

Protein biochips patterned by microcontact printing or by adsorption-soft lithography in two modes

  • Jens A. Wigenius1, 2,
  • Sophia Fransson1Email author,
  • Fredrik von Post1 and
  • Olle Inganäs1
Biointerphases3:30300075

https://doi.org/10.1116/1.2988771

Received: 30 May 2008

Accepted: 28 August 2008

Abstract

Patterning of proteins is critical to protein biochips. Printing of layers of proteins is well established, as is adsorption of proteins to surfaces properly modified with surface chemical functionalities. The authors show that simple methods based on soft lithography stamps can be used to prepare functional antibody chips through both these routes. Both methods incorporate transfer of the stamp material poly(dimethylsiloxane) (PDMS) to the biochip, whether intended or not intended. The results indicate that microcontact printing of proteins always includes PDMS transfer, thereby creating a possibility of unspecific adsorption to a hydrophobic domain.