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Journal for Biophysical Chemistry

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Imaging of G protein-coupled receptors in solid-supported planar lipid membranes

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This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a novel approach for the functional incorporation of membrane proteins, i.e., by their cell-free expression and in vitro reconstitution in the presence of tBLMs. By the combination of the corresponding coding DNA with the protein synthesis machinery of a cell-extract (in vitro transcription and translation), the authors observe spontaneous and vectorial insertions of an interesting example of the GPCR family into a tethered bimolecular lipid membrane: the olfactory receptor OR5. After synthesis, imaging of the surface area is performed and the resulting signals are analyzed in order to resolve quantity and lateral mobilities. Ligand-independent aggregation behavior of the GPCRs and quantitative analysis of the fluorescent signals are presented in this work.


  1. 1

    R. Robelek, F. D. Stefani, and W. Knoll, Phys. Status Solidi A 203, 3468 (2006).

  2. 2

    R. Robelek, E. S. Lemker, B. Wiltschi, V. Kirste, R. Naumann, D. Oesterhelt, and E.-K. Sinner, Angew. Chem., Int. Ed. 46, 605 (2007).

  3. 3

    S. Heyse, O. P. Ernst, Z. Dienes, K. P. Hofmann, and H. Vogel, Biochemistry 37, 507 (1998).

  4. 4

    C. Bieri, O. P. Ernst, S. Heyse, K. P. Hofmann, and H. Vogel, Nat. Biotechnol. 17, 1105 (1999).

  5. 5

    Y. Lill, K. L. Martinez, M. A. Lill, B. H. Meyer, H. Vogel, and B. Hecht, ChemPhysChem 6, 1633 (2005).

  6. 6

    N. Kahya and P. Schwille, Mol. Membr Biol. 23, 29 (2006).

  7. 7

    V. Jacquier, M. Prummer, J.-M. Segura, H. Pick, and H. Vogel, Proc.Natl. Acad. Sci. U.S.A. 103, 14325 (2006).

  8. 8

    J.-B. Perez, J.-M. Segura, D. Abankwa, J. Piguet, K. L. Martinez, and H. Vogel, J. Mol. Biol. 363, 918 (2006).

  9. 9

    B. Wiltschi, W. Knoll, and E.-K. Sinner, Methods 39, 134 (2006).

  10. 10

    N. O. Petersen, C. Brown, A. Kaminski, J. Rocheleau, M. Srivastava, and P. W. Wiseman, Faraday Discuss. 111, 289 (1999).

  11. 11

    M. Leutenegger and T. Lasser, Opt. Express 16, 8519 (2008).

  12. 12

    M. Leutenegger, “Single molecule detection on surfaces,” Ph.D. Thesis, EPFL, 2007,

  13. 13

    A. G. Benn and R. J. Kulperger, Environmetrics 7, 167 (1996).

  14. 14

    D. Marr and E. Hildreth, Proc. R. Soc. London, Ser. B 207, 187 (1980).

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