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Journal for Biophysical Chemistry

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Charging and structure of zwitterionic supported bilayer lipid membranes studied by streaming current measurements, fluorescence microscopy, and attenuated total reflection Fourier transform infrared spectroscopy

Biointerphases volume 4, pages 1–6 (2009)Cite this article

Abstract

The authors report on the characterization of the charge formation at supported bilayer lipid membranes (sBLMs) prepared from the zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine on planar silicon dioxide substrates. The charging of the sBLMs was studied in KCl solutions of different ionic strengths between 0.1 and 10 mM by streaming current measurements. In addition, attenuated total reflection Fourier transform infrared spectroscopy and fluorescence microscopy were applied to determine the lipid concentration in the membrane and to study the influence of the harsh conditions (pH 9-2, shear forces) during the electrokinetic measurements on the membrane stability and the lipid diffusion coefficient. The sBLMs were found to be extremely stable. Isoelectric points of about 4 revealed that unsymmetrical adsorption of hydroxide and hydronium ions determined the charging of the outer leaflet of the membrane in the investigated pH range. The diffusion coefficients were found to be rather independent on the ionic strength at neutral and alkaline pH. However, significantly decreased lipid diffusion at pH<4 indicated a charge-induced transition of the fluidic bilayer into a gel/ordered-phase bilayer.

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Authors and Affiliations

  1. Max Bergmann Center of Biomaterials Dresden, Leibniz Institute of Polymer Research Dresden, Hohe Strasse 6, 01069, Dresden, Germany

    Ralf Zimmermann, David Küttner, Lars Renner, Martin Kaufmann, Jan Zitzmann, Martin Müller & Carsten Werner

  2. Institute of Biomaterials and Biomedical Engineering, University of Toronto, 5 King’s College Road, M5S 3G8, Toronto, Ontario, Canada

    Carsten Werner

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  1. Ralf Zimmermann
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  2. David Küttner
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  3. Lars Renner
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  4. Martin Kaufmann
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  5. Jan Zitzmann
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  6. Martin Müller
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  7. Carsten Werner
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Zimmermann, R., Küttner, D., Renner, L. et al. Charging and structure of zwitterionic supported bilayer lipid membranes studied by streaming current measurements, fluorescence microscopy, and attenuated total reflection Fourier transform infrared spectroscopy. Biointerphases 4, 1–6 (2009). https://doi.org/10.1116/1.3082042

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  • DOI: https://doi.org/10.1116/1.3082042