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Journal for Biophysical Chemistry

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Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Biointerphases volume 4, pages 19–26 (2009)Cite this article

Abstract

Tethered bilayer lipid membranes are promising biomimetic architectures. Their formation has been investigated using four different surface sensitive techniques, including optical, acoustic, and electrical methods. The lipid bilayers are built in a two-step procedure; the proximal layer is formed by self-assembly and is then completed to a bilayer by fusion with small vesicles. The different technical approaches revealed specific aspects of the layer formation processes, namely, first a fast adsorption process followed by a longer rearrangement period. Similar phenomena have been observed for the vesicle fusion process. The results allow for a more controlled assembly protocol for the preparation of highly insulating lipid membranes.

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Authors and Affiliations

  1. Max Planck Institute for Polymer Research, 55021, Mainz, Germany

    Inga K. Vockenroth & Ingo Köper

  2. University of Bath, BA2 7AY, Bath, UK

    Inga K. Vockenroth

  3. Université de Technologie, 60200, Compiègne, France

    Claire Rossi

  4. HEJ Research Institute of Chemistry, University of Karachi, 75270, Karachi, Pakistan

    Muhammad Raza Shah

Authors
  1. Inga K. Vockenroth
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  2. Claire Rossi
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  3. Muhammad Raza Shah
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  4. Ingo Köper
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Correspondence to Ingo Köper.

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Vockenroth, I.K., Rossi, C., Shah, M.R. et al. Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques. Biointerphases 4, 19–26 (2009). https://doi.org/10.1116/1.3122019

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  • DOI: https://doi.org/10.1116/1.3122019