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Journal for Biophysical Chemistry

Bio-organism sensing via surface enhanced Raman spectroscopy on controlled metal/polymer nanostructured substrates

Abstract

A new class of nonlithographically prepared surface enhanced Raman spectroscopy (SERS) substrates based on metalized, nanostructured poly(p-xylylene) films has been developed and optimized for surface plasmon response with a view to applications of SERS detection of microbial pathogens, specifically, bacteria and viruses. The main emphasis has been on achieving high spot to spot, sample to sample reproducibility of the SERS signals while maintaining useful enhancement factors. The use of these surfaces, metalized with either Ag or Au, provides a noninvasive and nondestructive method for spectral fingerprint analyses of both bacteria and viruses. Examples are given for the detection of bacteria (E. coli and B. cereus) and viruses (respiratory syncytial virus and Coxsackievirus). Our method is able to distinguish Gram positive from Gram negative bacterial strains as well as enveloped and nonenveloped viruses. The results demonstrate the development of a new class of SERS substrates which can provide rapid, selective identification of infectious agents without amplification of cultures.

References

  1. P. Yager, G. J. Domingo, and J. Gerdes, Annu. Rev. Biomed. Eng. 10, 107 (2008).

    Article  CAS  Google Scholar 

  2. J. Kneipp, H. Kneipp, and K. Kneipp, Chem. Soc. Rev. 37, 1052 (2008).

    Article  CAS  Google Scholar 

  3. P. L. Stiles, J. A. Dieringer, N. C. Shah, and R. R. Van Duyne, Annu. Rev. Anal. Chem. 1, 601 (2008).

    Article  CAS  Google Scholar 

  4. K. A. Hartman, N. Clayton, and G. J. Thomas, Biochem. Biophys. Res. Commun. 50, 942 (1973).

    Article  CAS  Google Scholar 

  5. M. G. Albrecht and J. A. Creighton, J. Am. Chem. Soc. 99, 5215 (1977).

    Article  CAS  Google Scholar 

  6. D. L. Jeanmaire and R. P. Vanduyne, J. Electroanal. Chem. 84, 1 (1977).

    Article  CAS  Google Scholar 

  7. M. Moskovits, L. L. Tay, J. Yang, and T. Haslett, Optical Properties of Nanostructured Random Media (Springer-Verlag, Berlin, Germany, 2002), Vol. 82, p. 215.

    Google Scholar 

  8. A. M. Stacy and R. P. Vanduyne, Chem. Phys. Lett. 102, 365 (1983).

    Article  CAS  Google Scholar 

  9. I. Pockrand, Surface Enhanced Raman Vibrational Studies at Solid/Gas Interfaces (Springer, Berlin, 1984).

    Google Scholar 

  10. U. Kreibig, B. Schmitz, and H. D. Breuer, Phys. Rev. B 36, 5027 (1987).

    Article  Google Scholar 

  11. K. Kneipp, H. Kneipp, V. B. Kartha, R. Manoharan, G. Deinum, I. Itzkan, R. R. Dasari, and M. S. Feld, Phys. Rev. E 57, R6281 (1998).

    Article  CAS  Google Scholar 

  12. S. Farquharson, L. Grigely, V. Khitrov, W. Smith, J. F. Sperry, and G. Fenerty, J. Raman Spectrosc. 35, 82 (2004).

    Article  CAS  Google Scholar 

  13. N. Peica, I. Pavel, S. C. Pinzaru, V. K. Rastogi, and W. Kiefer, J. Raman Spectrosc. 36, 657 (2005).

    Article  CAS  Google Scholar 

  14. M. Kahraman, M. M. Yazici, F. Sahin, O. F. Bayrak, and M. Culha, Appl. Spectrosc. 61, 479 (2007).

    Article  CAS  Google Scholar 

  15. P. F. Liao, J. G. Bergman, D. S. Chemla, A. Wokaun, J. Melngailis, A. M. Hawryluk, and N. P. Economou, Chem. Phys. Lett. 82, 355 (1981).

    Article  CAS  Google Scholar 

  16. N. Felidj, J. Aubard, G. Levi, J. R. Krenn, M. Salerno, G. Schider, B. Lamprecht, A. Leitner, and F. R. Aussenegg, Phys. Rev. B 66, 245407 (2002).

    Article  Google Scholar 

  17. C. L. Haynes and R. P. Van Duyne, J. Phys. Chem. B 105, 5599 (2001).

    Article  CAS  Google Scholar 

  18. M. D. Musick, C. D. Keating, L. A. Lyon, S. L. Botsko, D. J. Pena, W. D. Holliway, T. M. McEvoy, J. N. Richardson, and M. J. Natan, Chem. Mater. 12, 2869 (2000).

    Article  CAS  Google Scholar 

  19. R. A. Tripp, R. A. Dluhy, and Y. P. Zhao, Nanotoday 3, 31 (2008).

    CAS  Google Scholar 

  20. M. C. Demirel, Colloids Surf., A 321, 121 (2008).

    Article  CAS  Google Scholar 

  21. M. C. Demirel, M. Cetinkaya, A. Singh, and W. J. Dressick, Adv. Mater. (Weinheim, Ger.) 19, 4495 (2007).

    Article  CAS  Google Scholar 

  22. S. Boduroglu, M. Cetinkaya, W. J. Dressick, A. Singh, and M. C. Demirel, Langmuir 23, 11391 (2007).

    Article  CAS  Google Scholar 

  23. A. Cetinkaya, S. Boduroglu, and M. C. Demirel, Polymer 48, 4130 (2007).

    Article  CAS  Google Scholar 

  24. P. Kao, N. Malvadkar, H. Wang, D. L. Allara, and M. C. Demirel, Adv. Mater. (Weinheim, Ger.) 20, 3562 (2008).

    Article  CAS  Google Scholar 

  25. M. Cetinkaya, N. Malvadkar, and M. C. Demirel, J. Polym. Sci., Part B: Polym. Phys. 46, 640 (2008).

    Article  CAS  Google Scholar 

  26. G. Mie, Ann. Phys. 25, 377 (1908).

    Article  CAS  Google Scholar 

  27. W. R. Premasiri, D. T. Moir, M. S. Klempner, N. Krieger, G. Jones, and L. D. Ziegler, J. Phys. Chem. B 109, 312 (2005).

    Article  CAS  Google Scholar 

  28. R. M. Jarvis and R. Goodacre, Anal. Chem. 76, 40 (2004).

    Article  CAS  Google Scholar 

  29. K. C. Schuster, I. Reese, E. Urlaub, J. R. Gapes, and B. Lendl, Anal. Chem. 72, 5529 (2000).

    Article  CAS  Google Scholar 

  30. P. D. Bao, T. Q. Huang, X. M. Liu, and T. Q. Wu, J. Raman Spectrosc. 32, 227 (2001).

    Article  CAS  Google Scholar 

  31. S. Shanmukh, L. Jones, J. Driskell, Y. P. Zhao, R. Dluhy, and R. A. Tripp, Nano Lett. 6, 2630 (2006).

    Article  CAS  Google Scholar 

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Demirel, M.C., Kao, P., Malvadkar, N. et al. Bio-organism sensing via surface enhanced Raman spectroscopy on controlled metal/polymer nanostructured substrates. Biointerphases 4, 35–41 (2009). https://doi.org/10.1116/1.3147962

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  • DOI: https://doi.org/10.1116/1.3147962