Biointerphases

Journal for Biophysical Chemistry

Biointerphases Cover Image
Open Access

Novel application of imaging surface plasmon resonance for in situ studies of the surface exploration of marine organisms

  • Olof Andersson1,
  • Tobias Ekblad1,
  • Nick Aldred2,
  • Anthony S. Clare2 and
  • Bo Liedberg3Email author
Biointerphases4:40400065

https://doi.org/10.1116/1.3274060

Received: 26 October 2009

Accepted: 17 November 2009

Abstract

The surface interactions of exploring cyprids of the barnacle Semibalanus balanoides were studied in situ using imaging surface plasmon resonance. It was demonstrated how the deposition of a proteinaceous adhesive could be followed in real time as the cyprids explored and temporarily attached to a surface. Furthermore, the amount of protein left on the surface when the cyprids moved on could be quantified. Clear differences were demonstrated between an oligo(ethyleneglycol) coated surface and a bare gold substrate. It is anticipated that this technique will be a valuable tool in the development of novel surface chemistries that can prevent biofouling.