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Journal for Biophysical Chemistry

Biointerphases Cover Image
  • Open Access

Novel application of imaging surface plasmon resonance for in situ studies of the surface exploration of marine organisms

  • 1,
  • 1,
  • 2,
  • 2 and
  • 3Email author

  • Received: 26 October 2009
  • Accepted: 17 November 2009


The surface interactions of exploring cyprids of the barnacle Semibalanus balanoides were studied in situ using imaging surface plasmon resonance. It was demonstrated how the deposition of a proteinaceous adhesive could be followed in real time as the cyprids explored and temporarily attached to a surface. Furthermore, the amount of protein left on the surface when the cyprids moved on could be quantified. Clear differences were demonstrated between an oligo(ethyleneglycol) coated surface and a bare gold substrate. It is anticipated that this technique will be a valuable tool in the development of novel surface chemistries that can prevent biofouling.