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Biointerphases

Journal for Biophysical Chemistry

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Assembly and structure of α-helical peptide films on hydrophobic fluorocarbon surfaces

Biointerphases volume 5, pages 9–16 (2010)Cite this article

Abstract

The structure, orientation, and formation of amphiphilic α-helix model peptide films on fluorocarbon surfaces has been monitored with sum frequency generation (SFG) vibrational spectroscopy, near-edge x-ray absorption fine structure (NEXAFS) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The α-helix peptide is a 14-mer of hydrophilic lysine and hydrophobic leucine residues with a hydrophobic periodicity of 3.5. This periodicity yields a rigid amphiphilic peptide with leucine and lysine side chains located on opposite sides. XPS composition analysis confirms the formation of a peptide film that covers about 75% of the surface. NEXAFS data are consistent with chemically intact adsorption of the peptides. A weak linear dichroism of the amide π is likely due to the broad distribution of amide bond orientations inherent to the α-helical secondary structure. SFG spectra exhibit strong peaks near 2865 and 2935 cm−1 related to aligned leucine side chains interacting with the hydrophobic surface. Water modes near 3200 and 3400 cm−1 indicate ordering of water molecules in the adsorbed-peptide fluorocarbon surface interfacial region. Amide I peaks observed near 1655 cm−1 confirm that the secondary structure is preserved in the adsorbed peptide. A kinetic study of the film formation process using XPS and SFG showed rapid adsorption of the peptides followed by a longer assembly process. Peptide SFG spectra taken at the air-buffer interface showed features related to well-ordered peptide films. Moving samples through the buffer surface led to the transfer of ordered peptide films onto the substrates.

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Author notes

  1. Newton T. Samuel

    Present address: CIBA VISION Corp., 30097, Duluth, GA

Authors and Affiliations

  1. Department of Bioengineering and Department of Chemical Engineering, National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, 98195, Seattle, Washington

    Tobias Weidner, Newton T. Samuel, Lara J. Gamble & David G. Castner

  2. The Polymer Technology Group, 94710, Berkeley, California

    Keith McCrea & Robert S. Ward

Authors
  1. Tobias Weidner
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  2. Newton T. Samuel
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  3. Keith McCrea
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  4. Lara J. Gamble
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  5. Robert S. Ward
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  6. David G. Castner
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Correspondence to David G. Castner.

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Weidner, T., Samuel, N.T., McCrea, K. et al. Assembly and structure of α-helical peptide films on hydrophobic fluorocarbon surfaces. Biointerphases 5, 9–16 (2010). https://doi.org/10.1116/1.3317116

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  • DOI: https://doi.org/10.1116/1.3317116