Biointerphases

Journal for Biophysical Chemistry

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Open Access

Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

  • Andreas O. Hohner1,
  • Maria Pamela C. David1 and
  • Joachim O. Rädlera1
Biointerphases5:50100001

https://doi.org/10.1116/1.3319326

Received: 30 October 2009

Accepted: 11 January 2010

Abstract

Controlled deposition of lipid bilayers plays a key role in creating supported membranes for biosensing devices and biophysical cell studies. The authors adopt a solvent-exchange method in order to deposit a phospholipid bilayer on solid substrates. The basic concept of deposition is to dissolve phospholipids in isopropanol-water mixtures and to increase water content gradually. Shortly before the onset of the micelle-to-vesicle transition, a lipid bilayer nucleates at the solid surface. They investigate the bulk phase behavior and surface coverage using small angle x-ray scattering and attenuated total reflection-Fourier transform infrared spectroscopy. They find a sequence of transitions from inverted-monomeric-micellar and vesicle phases correlating with an increasing amount of lipid on the adsorption layer. Supported lipid bilayers, prepared using this approach, are homogeneous and fluid.