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Journal for Biophysical Chemistry

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Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Biointerphases volume 5, pages 1–8 (2010)Cite this article

Abstract

Controlled deposition of lipid bilayers plays a key role in creating supported membranes for biosensing devices and biophysical cell studies. The authors adopt a solvent-exchange method in order to deposit a phospholipid bilayer on solid substrates. The basic concept of deposition is to dissolve phospholipids in isopropanol-water mixtures and to increase water content gradually. Shortly before the onset of the micelle-to-vesicle transition, a lipid bilayer nucleates at the solid surface. They investigate the bulk phase behavior and surface coverage using small angle x-ray scattering and attenuated total reflection-Fourier transform infrared spectroscopy. They find a sequence of transitions from inverted-monomeric-micellar and vesicle phases correlating with an increasing amount of lipid on the adsorption layer. Supported lipid bilayers, prepared using this approach, are homogeneous and fluid.

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Authors and Affiliations

  1. Centre for Nanoscience and Fakultät für Physik, Ludwig-Maximilians-Universität, Geschwister-Scholl-Platz 1, D-80539, München, Germany

    Andreas O. Hohner, Maria Pamela C. David & Joachim O. Rädlera

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  1. Andreas O. Hohner
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  2. Maria Pamela C. David
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  3. Joachim O. Rädlera
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Hohner, A.O., David, M.P.C. & Rädlera, J.O. Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces. Biointerphases 5, 1–8 (2010). https://doi.org/10.1116/1.3319326

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  • DOI: https://doi.org/10.1116/1.3319326