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Biointerphases

Journal for Biophysical Chemistry

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Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum

Biointerphases volume 5, pages 30–36 (2010)Cite this article

Abstract

Protein resistant oligo(ethylene glycol) (OEG) terminated self-assembled monolayers SAMs) of thiols on gold are commonly used for suppression of nonspecific protein adsorption in biology and biotechnology. The standard preparation for these SAMs is the solution method (SM) that involves immersion of the gold surface in an OEG solution. Here the authors present the preparation of 11-(mercaptoundecyl)-triethylene glycol [HS(CH2)11(OCH2CH2)3OH] SAMs on gold surface by vapor deposition (VD) in vacuum. They compare the properties of SAMs prepared by VD and SM using x-ray photoelectron spectroscopy (XPS), polarization modulation infrared reflection absorption spectroscopy, and surface plasmon resonance measurements. VD and SM SAMs exhibit similar packing density and show a similar resistance to the nonspecific adsorption of various proteins bovine serum albumin, trypsin, and myoglobin) under physiological conditions. A very high sensitivity of the OEG SAMs to x-ray radiation is found, which allows tuning their protein resistance. These results show a new path to in situ engineering, analysis, and patterning of protein resistant OEG SAMs by high vacuum and ultrahigh vacuum techniques.

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Authors and Affiliations

  1. Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld, 33615, Bielefeld, Germany

    Laxman Kankate, Udo Werner, Andrey Turchanin & Armin Gölzhäuser

  2. Institute of Biochemistry, Biocenter, Cluster of Excellence-Macromolecular Complexes, Johann Wolfgang Goethe-University, 60438, Frankfurt, Germany

    Helge Großmann & Robert Tampé

Authors
  1. Laxman Kankate
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  2. Udo Werner
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  3. Andrey Turchanin
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  4. Armin Gölzhäuser
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  5. Helge Großmann
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  6. Robert Tampé
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Kankate, L., Werner, U., Turchanin, A. et al. Protein resistant oligo(ethylene glycol) terminated self-assembled monolayers of thiols on gold by vapor deposition in vacuum. Biointerphases 5, 30–36 (2010). https://doi.org/10.1116/1.3407483

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  • DOI: https://doi.org/10.1116/1.3407483