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Biointerphases

Journal for Biophysical Chemistry

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UV laser-ablated surface textures as potential regulator of cellular response

Biointerphases volume 5, pages 53–59 (2010)Cite this article

Abstract

Textured surfaces obtained by UV laser ablation of poly(ethylene terephthalate) films were used to study the effect of shape and spacing of surface features on cellular response. Two distinct patterns, cones and ripples with spacing from 2 to 25 μm, were produced. Surface features with different shapes and spacings were produced by varying pulse repetition rate, laser fluence, and exposure time. The effects of the surface texture parameters, i.e., shape and spacing, on cell attachment, proliferation, and morphology of neonatal human dermal fibroblasts and mouse fibroblasts were studied. Cell attachment was the highest in the regions with cones at ≈4 μm spacing. As feature spacing increased, cell spreading decreased, and the fibroblasts became more circular, indicating a stress-mediated cell shrinkage. This study shows that UV laser ablation is a useful alternative to lithographic techniques to produce surface patterns for controlling cell attachment and growth on biomaterial surfaces.

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Author information

Author notes

  1. Karen Lai

    Present address: Duke University School of Medicine, Durham, 27701, Durham, NC, USA

  2. Hak-Joon Sung

    Present address: Department of Biomedical Engineering, Vanderbilt University, 5824 Stevenson Center, 37235, Nashville, TN, USA

Authors and Affiliations

  1. New Jersey Center for Biomaterials, Rutgers University, 145 Bevier Road, 08854, Piscataway, New Jersey, USA

    Prafulla Chandra, Karen Lai, Hak-Joon Sung, N. Sanjeeva Murthy & Joachim Kohn

Authors
  1. Prafulla Chandra
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  2. Karen Lai
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  3. Hak-Joon Sung
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  4. N. Sanjeeva Murthy
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  5. Joachim Kohn
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Correspondence to Joachim Kohn.

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Chandra, P., Lai, K., Sung, HJ. et al. UV laser-ablated surface textures as potential regulator of cellular response. Biointerphases 5, 53–59 (2010). https://doi.org/10.1116/1.3438080

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  • DOI: https://doi.org/10.1116/1.3438080