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Journal for Biophysical Chemistry

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Cationized albumin-biocoatings for the immobilization of lipid vesicles

Biointerphases volume 5, pages FA78–FA87 (2010)Cite this article

Abstract

Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions—bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin (cBSA) blood plasma proteins (cBSA-113 and cBSA-147). Quantitative analysis of protein adsorption was performed as the cBSA coatings were characterized by atomic force microscopy, surface zeta potential measurement, fluorescence microscopy, and surface plasmon spectroscopy, revealing a maximal surface coverage 270–280 ng/cm2 for 0.02 mg/ml cBSA on gold. Small unilamellar vesicles as well as giant unilamellar vesicles (GUVs) were readily immobilized (∼15 min) on cBSA coated surfaces. GUVs with 5–10 mol% negatively charged 1,2,-dipalmitoyl-sn-glycero-3-phosphoglycerol remained stable in liquid for at least 5 weeks.

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Authors and Affiliations

  1. Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany

    Sandra Ritz, Klaus Eisele, Jan Dorn, Shaohua Ding, Doris Vollmer, Sabine Pütz & Tanja Weil

  2. Molecular Biophysics, Johannes Gutenberg University, D-55128, Mainz, Germany

    Sandra Ritz & Jan Dorn

  3. Institute of Organic Chemistry III, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany

    Klaus Eisele & Tanja Weil

  4. Max Planck Institute for Polymer Research, Ackermannweg 10, D-55128, Mainz, Germany

    Tanja Weil

  5. Department of Chemistry, National University of Singapore, 3 Science Drive, 117543, Singapore, Republic of Singapore

    Eva-Kathrin Sinner

  6. Institute of Materials Research and Engineering (IMRE), 3 Research Link, 117602, Singapore, Republic of Singapore

    Eva-Kathrin Sinner

Authors
  1. Sandra Ritz
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  2. Klaus Eisele
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  3. Jan Dorn
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  4. Shaohua Ding
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  5. Doris Vollmer
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  6. Sabine Pütz
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  7. Tanja Weil
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  8. Eva-Kathrin Sinner
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Additional information

This paper is part of an In Focus section on Biointerface Science in Singapore, sponsored by Bruker Optik Southeast Asia, IMRE, the Provost's Office and School of Materials Science and Engineering of Nanyang Technological University, and Analytical Technologies Pte. Ltd.

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Ritz, S., Eisele, K., Dorn, J. et al. Cationized albumin-biocoatings for the immobilization of lipid vesicles. Biointerphases 5, FA78–FA87 (2010). https://doi.org/10.1116/1.3494039

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  • DOI: https://doi.org/10.1116/1.3494039