Biointerphases

Journal for Biophysical Chemistry

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Open Access

Cationized albumin-biocoatings for the immobilization of lipid vesicles

  • Sandra Ritz1, 2,
  • Klaus Eisele1, 3,
  • Jan Dorn1, 2,
  • Shaohua Ding1,
  • Doris Vollmer1,
  • Sabine Pütz1,
  • Tanja Weil1, 3, 4 and
  • Eva-Kathrin Sinner5, 6
Biointerphases20105:50300078

https://doi.org/10.1116/1.3494039

Received: 9 July 2010

Accepted: 1 September 2010

Published: 14 December 2010

Abstract

Tethered lipid membranes or immobilized lipid vesicles are frequently used as biomimetic systems. In this article, the authors presented a suitable method for efficient immobilization of lipid vesicles onto a broad range of surfaces, enabling analysis by quantitative methods even under rigid, mechanical conditions—bare surfaces such as hydrophilic glass surfaces as well as hydrophobic polymer slides or metal surfaces such as gold. The immobilization of vesicles was based on the electrostatic interaction of zwitterionic or negatively charged lipid vesicles with two types of cationic chemically modified bovine serum albumin (cBSA) blood plasma proteins (cBSA-113 and cBSA-147). Quantitative analysis of protein adsorption was performed as the cBSA coatings were characterized by atomic force microscopy, surface zeta potential measurement, fluorescence microscopy, and surface plasmon spectroscopy, revealing a maximal surface coverage 270–280 ng/cm2 for 0.02 mg/ml cBSA on gold. Small unilamellar vesicles as well as giant unilamellar vesicles (GUVs) were readily immobilized (15 min) on cBSA coated surfaces. GUVs with 5–10 mol% negatively charged 1,2,-dipalmitoyl-sn-glycero-3-phosphoglycerol remained stable in liquid for at least 5 weeks.

Notes