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Current development of bioreactors for extracorporeal bioartificial liver (Review)
Biointerphases volume 5, pages FA116–FA131 (2010)
Abstract
The research and development of extracorporeal bioartificial liver is gaining pace in recent years with the introduction of a myriad of optimally designed bioreactors with the ability to maintain long-term viability and liver-specific functions of hepatocytes. The design considerations for bioartificial liver are not trivial; it needs to consider factors such as the types of cell to be cultured in the bioreactor, the bioreactor configuration, the magnitude of fluid-induced shear stress, nutrients' supply, and wastes' removal, and other relevant issues before the bioreactor is ready for testing. This review discusses the exciting development of bioartificial liver devices, particularly the various types of cell used in current reactor designs, the state-of-the-art culturing and cryopreservation techniques, and the comparison among many today's bioreactor configurations. This review will also discuss in depth the importance of maintaining optimal mass transfer of nutrients and oxygen partial pressure in the bioreactor system. Finally, this review will discuss the commercially available bioreactors that are currently undergoing preclinical and clinical trials.
Reference
S. M. Riordan and R. Williams, Semin Liver Dis. 2, 28 (2008).
V. Stadlbauer, N. A. Davies, S. Sen, and R. Jalan, Semin Liver Dis. 28, 096 (2008).
H. I. Pryor II and J. P. Vacanti, Front. Biosci. 1, 13 (2008).
4J. Phua and K. H. Lee, Curr. Opin. Crit. Care 2, 14 (2008).
B. Carpentier, A. Gautier, and C. Legallais, Gut 58, 1690 (2009).
I. H. Borel Rinkes, M. Toner, S. J. Sheeha, R. G. Tompkins, and M. L. Yarmush, Cell Transplant 1, 281 (1992).
M. P. van de Kerkhove, R. Hoekstra, R. A. Chamuleau, and T. M. van Gulik, Ann. Surg. 240, 216 (2004).
P. P. Poyck, R. Hoekstra, A. C. van Wijk, C. Attanasio, F. Calise, R. A. Chamuleau, and T. M. van Gulik, Liver Transpl. 13, 589 (2007).
U. Baccarani et al., Liver Transpl. 9, 506 (2003).
R. D. Hughes, R. R. Mitry, A. Dhawan, S. C. Lehec, R. Girlanda, M. Rela, N. D. Heaton, and P. Muiesan, Liver Transpl. 5, 12 (2006).
A. Baquerizo, A. Mhoyan, M. Kearns-Jonker, W. S. Arnaout, C. Shackleton, R. W. Busuttil, A. A. Demetriou, and D. V. Cramer, Transplantation 67, 5 (1999).
J. Schultearn Esch et al., Transplant. Proc. 34, 2321 (2002).
M. P. van de Kerkhove, M. R. Germans, T. Deurholt, R. Hoekstra, D. H. Joziasse, C. W. A. van Wijk, T. M. van Gulik, R. A. F. M. Chamuleau, and A. Roos, J. Hepatol 42, 541 (2005).
H. Hasegawa, M. Shimada, T. Gion, H. Ijima, K. Nakazawa, K. Funatsu, and K. Sugimachi, ASAIO J. 45, 392 (1999).
L. Lai et al., Science 295, 1089 (2002).
T. Fujimura, Y. Takahagi, T. Shigehisa, H. Nagashima, S. Miyagawa, R. Shirakura, and H. Murakami, Mol. Reprod. Dev. 75, 1372 (2008).
S. Harrison et al., Cloning Stem Cells 6, 327 (2004).
Z. Pitkin and C. Mullon, Artif. Organs 23, 829 (1999).
J. H. Blusch, C. Patience, Y. Takeuchi, C. Templin, C. Roos, K. Von Der Helm, G. Steinhoff, and U. Martin, J. Virol. 74, 7687 (2000).
V. Specke, R. Plesker, J. Wood, C. Coulibaly, K. Suling, C. Patience, R. Kurth, H. J. Schuurman, and J. Denner, Xenotransplantation 16, 34 (2009).
B. W. McKane, S. Ramachandran, X. C. Xu, B. J. Olack, W. C. Chapman, and T. Mohanakumar, Cell Transplant 13, 137 (2004).
R. Kuddus, J. F. PatzerII, R. Lopez, G. V. Mazariegos, B. Meighen, D. J. Kramer, and A. S. Rao, Transplantation 73, 420 (2002).
S. Miyagawa et al., J. Biochem. 137, 503 (2005).
B. Dieckhoff, A. Karlas, A. Hofmann, W. A. Kues, B. Petersen, A. Pfeifer, H. Niemann, R. Kurth, and J. Denner, Arch. Virol. 152, 629 (2007).
J. Denner, Transplant. Proc. 40, 587 (2008).
C. A. Wilson, Cell. Mol. Life Sci. 65, 3399 (2008).
S. H. Qari, S. Magre, J. G. Garcia-Lerma, A. I. Hussain, Y. Takeuchi, C. Patience, R. A. Weiss, and W. Heneine, J. Virol. 75, 1048 (2001).
S. K. Powell, M. E. Gates, G. Langford, M. L. Gu, C. Lockey, Z. Long, and E. Otto, Antimicrob. Agents Chemother. 44, 3432 (2000).
M. Wilhelm, J. A. Fishman, R. Pontikis, A. M. Aubertin, and F. X. Wilhelm, Cell. Mol. Life Sci. 59, 2184 (2002).
S. Dekker, W. Toussaint, G. Panayotou, T. de Wit, P. Visser, F. Grosveld, and D. Drabek, J. Virol. 77, 12132 (2003).
L. Wang, J. Sun, L. Li, D. Mears, M. Horvat, and A. G. Sheil, Cell Transplant 7, 459 (1998).
D. Mavri-Damelin, L. H. Damelin, S. Eaton, M. Rees, C. Selden, and H. J. Hodgson, Biotechnol. Bioeng. 99, 644 (2008).
R. J. Thomas, R. Bhandari, D. A. Barrett, A. J. Bennett, J. R. Fry, D. Powe, B. J. Thomson, and K. M. Shakesheff, Cells Tissues Organs 181, 67 (2005).
Y. S. Zinchenko, C. R. Culberson, and R. N. Coger, Tissue Eng. 12, 2241 (2006).
Y. S. Zinchenko, L. W. Schrum, M. Clemens, and R. N. Coger, Tissue Eng. 12, 751 (2006).
M. Nishikawa, N. Kojima, K. Komori, T. Yamamoto, T. Fujii, and Y. Sakai, J. Biotechnol. 133, 253 (2008).
M. Ohno, K. Motojima, T. Okano, and A. Taniguchi, Tissue Eng Part A 14, 1861 (2008).
S. M. Chia, P. C. Lin, and H. Yu, Biotechnol. Bioeng. 89, 565 (2005).
M. Ohno, K. Motojima, T. Okano, and A. Taniguchi, J. Biochem. 145, 591 (2009).
S. R. Khetani, G. Szulgit, J. A. Del Rio, C. Barlow, and S. N. Bhatia, Hepatology 40, 545 (2004).
S. R. Khetani, A. A. Chen, B. Ranscht, and S. N. Bhatia, FASEB J. 22, 3768 (2008).
H. Hang, X. Shi, G. X. Gu, Y. Wu, J. Gu, and Y. Ding, Liver Int. 30, 611 (2010).
H. Hang, X. Shi, G. Gu, Y. Wu, and Y. Ding, Int. J. Artif. Organs 32, 720 (2009).
C. Terry, A. Dhawan, R. R. Mitry, S. C. Lehec, and R. D. Hughes, Liver Transpl. 12, 165 (2006).
X. Stephenne, M. Najimi, and E. M. Sokal, World J. Gastroenterol. 16, 1 (2009).
Y. Miyamoto, S. Suzuki, K. Nomura, and S. Enosawa, Cell Transplant 15, 911 (2006).
E. Katenz, F. W. Vondran, R. Schwartlander, G. Pless, X. Gong, X. Cheng, P. Neuhaus, and I. M. Sauer, Liver Transpl. 13, 38 (2007).
L. S. Limaye and V. P. Kale, J. Hematother. Stem Cell Res. 10, 709 (2001).
L. M. Sasnoor, V. P. Kale, and L. S. Limaye, J. Hematother. Stem Cell Res. 12, 553 (2003).
T. Fu, D. Guo, X. Huang, M. R. O'Gorman, L. Huang, S. E. Crawford, and H. E. Soriano, Cell Transplant 10, 59 (2001).
V. P. Vanhulle, A. M. Neyrinck, J. M. Pycke, Y. Horsmans, and N. M. Delzenne, Life Sci. 78, 1570 (2006).
T. Hoshiba, H. Nagahara, C. S. Cho, Y. Tagawa, and T. Akaike, Biomaterials 28, 1093 (2007).
L. Wu, J. Sun, L. Wang, C. Wang, K. Woodman, N. Koutalistras, M. Horvat, and A. G. Sheil, Transplant. Proc. 32, 2271 (2000).
W. De Loecker, V. A. Koptelov, V. I. Grischenko, and P. De Loecker, Cryobiology 37, 103 (1998).
K. Yoshida, Y. Matsui, T. Wei, M. Kaibori, A. H. Kwon, A. Yamane, and Y. Kamiyama, J. Surg. Res. 81, 216 (1999).
J. V. Rodriguez, L. L. Almada, M. E. Mamprin, E. E. Guibert, and G. Furno, Ann. Hepatol. 8, 57 (2009).
A. Miskon, T. Yamaoka, S. H. Hyon, M. Kodama, and H. Uyama, Tissue Eng Part C Methods 15, 345 (2009).
L. L. Kuleshova, S. S. Gouk, and D. W. Hutmacher, Biomaterials 28, 1585 (2007).
M. Richards, C. Y. Fong, S. Tan, W. K. Chan, and A. Bongso, Stem Cells 22, 779 (2004).
T. Fujioka, K. Yasuchika, Y. Nakamura, N. Nakatsuji, and H. Suemori, Int. J. Dev. Biol. 48, 1149 (2004).
H. Suemori, K. Yasuchika, K. Hasegawa, T. Fujioka, N. Tsuneyoshi, and N. Nakatsuji, Biochem. Biophys. Res. Commun. 345, 926 (2006).
F. Wen, R. Magalhaes, S. S. Gouk, G. Bhakta, K. H. Lee, D. W. Hutmacher, and L. L. Kuleshova, Tissue Eng Part C Methods 15, 105 (2009).
R. Magalhães, P. R. Anil Kumar, F. Wen, X. Zhao, H. Yu, and L. L. Kuleshova, Biomaterials 30, 4136 (2009).
R. Magalhães, X. W. Wang, S. S. Gouk, K. H. Lee, C. M. Ten, H. Yu, and L. L. Kuleshova, Cell Transplant 17, 813 (2008).
Y. Wu, H. Yu, S. Chang, R. Magalhaes, and L. L. Kuleshova, Tissue Eng. 13, 649 (2007).
J. W. Allen and S. N. Bhatia, Biotechnol. Bioeng. 82, 253 (2003).
R. Gebhardt, Pharmacol. Ther. 53, 275 (1992).
K. Jungermann and T. Kietzmann, Hepatology 31, 255 (2000).
J. P. Sullivan, J. E. Gordon, T. Bou-Akl, H. W. Matthew, and A. F. Palmer, Artif. Cells Blood Substit Immobil Biotechnol. 35, 585 (2007).
E. M. Kekonen, V. P. Jauhonen, and I. E. Hassinen, J. Cell. Physiol. 133, 119 (1987).
R. M. Subramanian, N. Chandel, G. R. Budinger, and P. T. Schumacker, Hepatology 45, 455 (2007).
A. Boveris and B. Chance, Biochem. J. 134, 707 (1973).
W. Levin, A. Y. Lu, M. Jacobson, R. Kuntzman, J. L. Poyer, and P. B. McCay, Arch. Biochem. Biophys. 158, 842 (1973).
M. Miyazaki, K. Utsumi, and J. Sato, Exp. Cell Res. 182, 415 (1989).
S. A. Suleiman and J. B. Stevens, In Vitro Cell Dev. Biol. 23, 332 (1987).
76K. Yanagi, H. Miyoshi, and N. Ohshima, ASAIO J. 44, 5 (1998).
J. F. Patzer II, Artif. Organs 28, 83 (2004).
L. Custer and C. J. Mullon, Adv. Exp. Med. Biol. 454, 261 (1998).
M. Nishikawa, J. Uchino, M. Matsushita, M. Takahashi, K. Taguchi, M. Koike, H. Kamachi, and H. Kon, Artif. Organs 20, 169 (1996).
M. Miyazaki, L. Bai, S. Tsuboi, K. Seshimo, and M. Namba, Acta Med. Okayama 45, 441 (1991).
A. Badera, N. Frühaufa, M. Tiedgeb, M. Drinkgernb, L. D. Bartoloc, J. T. Borlaka, G. Steinhoffa, and A. Havericha, Exp. Cell Res. 246, 221 (1999).
P. D. Hay, A. R. Veitch, and J. D. Gaylor, Artif. Organs 25, 119 (2001).
M. Wolff, J. Fandrey, and W. Jelkmann, J. Am. Physiol. 265, C1266 (1993).
J. W. Boag, Int. J. Radiat. Biol. 18, 475 (1970).
P. T. Schumacker, N. Chandel, and A. G. Agusti, Am. J. Physiol. 265, L395 (1993).
J. G. Conway, F. C. Kauffman, S. Ji, and R. G. Thurman, Mol. Pharmacol. 22, 509 (1982).
T. Kashiwagura, D. F. Wilson, and M. Erecinska, J. Cell. Physiol. 120, 13 (1984).
K. Hayashi, T. Ochiai, Y. Ishinoda, T. Okamoto, T. Maruyama, K. Tsuda, and H. Tsubouchi, J. Gastroenterol. Hepatol 12, 3 (1997).
S. T. Koury, M. C. Bondurant, M. J. Koury, and G. L. Semenza, Blood 77, 2497 (1991).
S. N. Bhatia, M. Toner, B. D. Foy, A. Rotem, K. M. O'Neil, R. G. Tompkins, and M. L. Yarmush, Journal of Cellular Engineering 1, 125 (1996).
S. H. Lin, J. Theor. Biol. 60, 449 (1976).
U. Balis, K. Behnia, B. Dwarakanath, S. Bhatia, S. Sullivan, M. Yarmush, and M. Toner, Metab. Eng. 1, 49 (1999).
A. Rotem, M. Toner, R. G. Tompkins, and M. L. Yarmush, Biotechnol. Bioeng. 40, 1286 (1992).
K. Yanagi and N. Ohshima, Artif. Organs 25, 1525 (2001).
95R. D. Guarino, L. E. Dike, T. A. Haq, J. A. Rowley, J. B. Pitner, and M. R. Timmins, Biotechnol. Bioeng. 86, 7 (2004).
C. H. Cho, J. Park, D. Nagrath, A. W. Tilles, F. Berthiaume, M. Toner, and M. L. Yarmush, Biotechnol. Bioeng. 97, 188 (2007).
H. Nakatsuka, T. Sokabe, K. Yamamoto, Y. Sato, K. Hatakeyama, A. Kamiya, and J. Ando, Am. J. Physiol. Gastrointest. Liver Physiol. 291, G26 (2006).
J. M. Schoen, H. H. Wang, G. Y. Minuk, and W. W. Lautt, Nitric Oxide 5, 453 (2001).
99P. Kan, H. Miyoshi, K. Yanagi, and N. Ohshima, ASAIO J. 44, M441 (1998).
T. Torii, M. Miyazawa, and I. Koyama, Transplant. Proc. 37, 4575 (2005).
A. Tilles, H. Baskaran, P. Roy, M. Yarmush, and M. Toner, Biotechnol. Bioeng. 73, 379 (2001).
Y. Tanaka, M. Yamato, T. Okano, T. Kitamori, and K. Sato, Meas. Sci. Technol. 17, 3167 (2006).
J. C. Dunn, R. G. Tompkins, and M. L. Yarmush, Biotechnol. Prog. 7, 554 (1991).
K. A. Barbee, T. Mundel, R. Lal, and P. F. Davies, Am. J. Physiol. 268, 1765 (1995).
B. Geiger and A. Bershadsky, Cell 110, 139 (2002).
J. Y. Shyy and S. Chien, Circ. Res. 91, 769 (2002).
Y. Zeng, T.-S. Lee, P. Yu, and H.-T. Low, Int. Commun. Heat Mass Transfer 34, 217 (2007).
J. P. Sullivan, J. E. Gordon, and A. F. Palmer, Biotechnol. Bioeng. 93, 306 (2006).
F. Consolo, G. Fiore, S. Truscello, M. Caronna, U. Morbiducci, F. Montevecchi, and A. Redaelli, Tissue Eng Part A 15, 1 (2008).
J. Park, F. Berthiaume, M. Toner, M. L. Yarmush, and A. W. Tilles, Biotechnol. Bioeng. 90, 632 (2005).
A. J. Ellis, R. D. Hughes, J. A. Wendon, J. Dunne, P. G. Langley, J. H. Kelly, G. T. Gislason, N. L. Sussman, and R. Williams, Hepatology 6, 24 (1996).
G. V. Mazariegos et al., ASAIO J. 47, 471 (2001).
O. Detry, N. Arkadopoulos, P. Ting, E. Kahaku, F. D. Watanabe, J. Rozga, and A. A. Demetriou, Am. Surg. 65, 934 (1999).
M. P. van de Kerkhove et al., Int. J. Artif. Organs 25, 950 (2002).
I. M. Sauer, N. Obermeyer, D. Kardassis, T. Theruvath, and J. C. Gerlach, Ann. N.Y. Acad. Sci. 944, 320 (2001).
Y. L. Xue et al., World J. Gastroenterol. 7, 826 (2001).
Y. T. Ding, Y. D. Qiu, Z. Chen, Q. X. Xu, H. Y. Zhang, Q. Tang, and D. C. Yu, World J. Gastroenterol. 9, 829 (2003).
J. Liu, T. Song, W. Jiang, Y. Zhang, G. Lv, L. Zhao, G. Zhang, and L. Li, Conference Proceedings of the IEEE Engineering in Medicine and Biology Society 2009, 2009 (unpublished).
Y. Gao, N. Mu, X. P. Xu, and Y. Wang, World J. Gastroenterol. 11, 5468 (2005).
S. L. Nyberg, R. A. Shatford, W. D. Payne, W. S. Hu, and F. B. Cerra, Cytotechnology 10, 205 (1992).
I. Jasmund, A. Langsch, R. Simmoteit, and A. Bader, Biotechnol. Prog. 18, 686 (2002).
H. Mizumoto and K. Funatsu, Artif. Organs 28, 1 (2004).
T. Gion, M. Shimada, K. Shirabe, K. Nakazawa, H. Ijima, T. Matsushita, K. Funatsu, and K. Sugimachi, J. Surg. Res. 82, 2 (1999).
A. Kinasiewicz, A. Gautier, D. Lewinska, J. Bukowski, C. Legallais, and A. Werynski, Transplant. Proc. 39, 2911 (2007).
K. Hideki et al., Artif. Organs 31, 148 (2007).
K. Naruse, Y. Sakai, I. Nagashima, G. X. Jiang, M. Suzuki, and T. Muto, Int. J. Artif. Organs 19, 605 (1996).
E. Morsiani et al., Int. J. Artif. Organs 25, 192 (2002).
K. Yanagi, K. Ookawa, S. Mizuno, and N. Ohshima, ASAIO Trans. 35, 57 (1989).
E. Dore and C. Legallais, Ther. Apher. 3, 3 (1999).
G. D. Wells, M. M. Fisher, and M. V. Sefton, Biomaterials 14, 8 (1993).
Dixit, V. and G. Gitnick, Eur. J. Surg. Suppl. 582, 132 (1998).
H. Takabatake, N. Koide, and T. Tsuji, Artif. Organs 15, 2 (1991).
G. Ambrosino, S. M. Basso, S. Varotto, E. Zardi, A. Picardi, and D. F. D'Amico, Cell Transplant 14, 151 (2005).
P. V. Moghe, F. Berthiaume, R. M. Ezzell, M. Toner, R. G. Tompkins, and M. L. Yarmush, Biomaterials 17, 373 (1996).
J. Uchino et al., ASAIO Trans. 34, 4 (1988).
K. Taguchi, M. Matsushita, M. Takahashi, and J. Uchino, Artif. Organs 20, 186 (1996).
L. De Bartolo, G. Jarosch-Von Schweder, A. Haverich, and A. Bader, Biotechnol. Prog. 16, 102 (2000).
138J. Park, F. Berthiaume, M. Toner, M. L. Yarmush, and A. W. Tilles, Biotechnol. Bioeng. 90, 632 (2005).
L. Kim, Y. C. Toh, J. Voldman, and H. Yu, Lab Chip 7, 681 (2007).
P. J. Lee, P. J. Hung, and L. P. Lee, Biotechnol. Bioeng. 97, 1340 (2007).
E. Leclerc, Y. Sakai, and T. Fujii, Biotechnol. Prog. 20, 590 (2004).
A. A. Demetriou, J. Whiting, S. M. Levenson, N. R. Chowdhury, R. Schechner, S. Michalski, D. Feldman, and J. R. Chowdhury, Ann. Surg. 204, 259 (1986).
C. Mullon and Z. Pitkin, Expert Opin. Investig. Drugs 8, 229 (1999).
D. Samuel et al., Transplantation 73, 257 (2002).
A. A. Demetriou et al., Ann. Surg. 239, 660 (2004).
N. L. Sussman, G. T. Gislason, C. A. Conlin, and J. H. Kelly, Artif. Organs 18, 390 (1994).
N. L. Sussman and J. H. Kelly, Artif. Organs 17, 27 (1993).
J. M. Millis, D. C. Cronin, R. Johnson, H. Conjeevaram, C. Conlin, S. Trevino, and P. Maguire, Transplantation 74, 1735 (2002).
L. M. Flendrig, J. W. la Soe, G. G. Jorning, A. Steenbeek, O. T. Karlsen, W. M. Bovee, N. C. Ladiges, A. A. te Velde, and R. A. Chamuleau, J. Hepatol. 26, 1379 (1997).
P. P. Poyck, A. C. van Wijk, T. V. van der Hoeven, D. R. de Waart, R. A. Chamuleau, T. M. van Gulik, R. P. Oude Elferink, and R. Hoekstra, J. Hepatol. 48, 266 (2008).
M. P. van de Kerkhove, P. P. Poyck, A. C. van Wijk, D. Galavotti, R. Hoekstra, T. M. van Gulik, and R. A. Chamuleau, Int. J. Artif. Organs 28, 617 (2005).
M. P. van de Kerkhove, P. P. Poyck, T. Deurholt, R. Hoekstra, R. A. Chamuleau, and T. M. van Gulik, Dig. Surg. 22, 254 (2005).
G. Mareels, P. P. Poyck, S. Eloot, R. A. Chamuleau, and P. R. Verdonck, Ann. Biomed. Eng. 34, 1729 (2006).
J. F. Patzer II et al., Ann. N.Y. Acad. Sci. 875, 340 (1999).
G. V. Mazariegos et al., Am. J. Transplant. 2, 260 (2002).
J. C. Gerlach, Int. J. Artif. Organs 19, 645 (1996).
A. Mundt et al., Int. J. Artif. Organs 25, 542 (2002).
I. M. Sauer et al., Int. J. Artif. Organs 25, 1001 (2002).
I. M. Sauer et al., Xenotransplantation 10, 460 (2003).
E. Morsiani, M. Brogli, D. Galavotti, T. Bellini, D. Ricci, P. Pazzi, and A. C. Puviani, Artif. Organs 25, 740 (2001).
K. S. Suh, H. Lilja, Y. Kamohara, S. Eguchi, N. Arkadopoulos, T. Neuman, A. A. Demetriou, and J. Rozga, J. Surg. Res. 85, 243 (1999).
J. C. Gerlach et al., Int. J. Artif. Organs 24, 793 (2001).
L. M. Flendrig et al., Int. J. Artif. Organs 22, 701 (1999).
M. N. Sosef, L. S. Abrahamse, M. P. van de Kerkhove, R. Hartman, R. A. Chamuleau, and T. M. van Gulik, Transplantation 73, 204 (2002).
J. Gordon and A. F. Palmer, Artif. Cells Blood Substit Immobil Biotechnol. 33, 297 (2005).
A. Kinasiewicz, A. Smietanka, B. Gajkowska, and A. Werynski, Artif. Cells Blood Substit Immobil Biotechnol. 36, 525 (2008).
M. J. Nieuwoudt et al., Artif. Organs 29, 915 (2005).
C. Chan, F. Berthiaume, B. D. Nath, A. W. Tilles, M. Toner, and M. L. Yarmush, Liver Transpl. 10, 1331 (2004).
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This paper is part of an In Focus section on Biointerphase Science in Singapore, sponsored by Bruker Optik Southeast Asia, IMRE, the Provost's Office and School of Materials Science and Engineering of Nanyang Technological University, and Analytical Technologies Pte. Ltd.
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Wang, Y., Susando, T., Lei, X. et al. Current development of bioreactors for extracorporeal bioartificial liver (Review). Biointerphases 5, FA116–FA131 (2010). https://doi.org/10.1116/1.3521520
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DOI: https://doi.org/10.1116/1.3521520