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Biointerphases

Journal for Biophysical Chemistry

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Phase separation of phospholipid multilayers incorporated with cell penetrating peptides

Biointerphases volume 6, pages 73–78 (2011)Cite this article

Abstract

We used X-ray reflectivity to investigate the structures of phospholipid multilayers with transcription-activating-factor-derived peptide (TDP) as a function of the membrane charge density. Mixed phospholipid multilayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphoserine (DPPS) with different mixing ratios (C:S) were used to elucidate the various charge densities in a plasma membrane. We fixed the peptide/lipid molar ratio (P/L) and varied the DPPC/DPPS molar ratio in the mixed multilayer. In the pure DPPC multilayer, the incorporation of TDP had nearly no effect on the bilayer thickness of the mixed lipid multilayer. However, in the mixed DPPC/DPPS multilayer, the incorporation of TDP decreased the bilayer thickness, suggesting that the TDP peptide had a stronger interaction with DPPS than with DPPC and caused disorder in the lamellar structure. Combining this with the refined X-ray reflectivity (XR) data, we concluded that the TDP existed more in the headgroup region of the TDP-induced segregated DPPS in the mixed multilayer and caused significant membrane thinning.

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Authors and Affiliations

  1. Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Institute of Biological Interfaces, Sogang University, Seoul, 121-742, Korea

    Huaiyu Jing, Heesuk Kim, Dongjin Choi & Kwanwoo Shin

  2. Department of Physics, Soongsil University, Seoul, 156-743, Korea

    Dong Ryeol Lee

  3. Korea Atomic Energy Research Institute, Yuseong, Daejon, 305-600, Korea

    Jeong Soo Lee

  4. Pohang Accelerator Laboratory, POSTECH, Pohang, 790-784, Korea

    Chung-Jong Yu

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  1. Huaiyu Jing
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  2. Heesuk Kim
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  4. Dong Ryeol Lee
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  5. Jeong Soo Lee
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  7. Kwanwoo Shin
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Jing, H., Kim, H., Choi, D. et al. Phase separation of phospholipid multilayers incorporated with cell penetrating peptides. Biointerphases 6, 73–78 (2011). https://doi.org/10.1116/1.3602087

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  • DOI: https://doi.org/10.1116/1.3602087