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Journal for Biophysical Chemistry

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Efficient creation of cellular micropatterns with long-term stability and their geometric effects on cell behavior

Biointerphases volume 6, pages143–152(2011)Cite this article

Abstract

Cellular micropatterning with bio-adhesive and nonadhesive areas has attracted increasing interest for the precise design of cell-to-surface attachment in cell biology studies, tissue engineering, cell-based biosensors, biological assays, and drug development and screening. In this paper we describe a simple and efficient method to create a two-dimensional stable cellular microenvironment, which is based on (1) forming a protein-resistant oligo(ethylene glycol) methyl ether methacrylate polymer layer on the substrates via surface-initiated atom transfer radical polymerization; (2) placing a defined photomask on the substrate and exposing the substrate to ultraviolet light; and (3) immersing the patterned surface in a fibronectin solution to form cell-adhesive protein patterns in a cellresistant background. The resulting surfaces are tailored into cell-adhesive and cell-resistant regions. Three different types of cells (NIH-3T3, PC12, bone marrow-derived mesenchymal stem cells) are seeded on such patterned surfaces to form cellular patterns. The geometric effects on cell behavior are investigated. The long-term stability is tested by NIH-3T3 fibroblasts and mesenchymal stem cells and excellent retention of cellular patterns is observed. The strategy illustrated here offers an efficient way to create a stable, patterned cellular microenvironment, and could be employed in tissue engineering to study the effect of micropatterns on the proliferation and differentiation of cells, and in particular mesenchymal stem cells. © 2011 American Vacuum Society.

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Affiliations

  1. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, People's Republic of China

    Ning-Ping Huang, Hai Yu, Yan-Yan Wang & Jun-Cai Shi

  2. Laboratory of Developmental Genes and Human Diseases, Medical School of Southeast University, Nanjing, 210009, People's Republic of China

    Xi Mao

Authors
  1. Ning-Ping Huang
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  2. Hai Yu
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  3. Yan-Yan Wang
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  4. Jun-Cai Shi
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  5. Xi Mao
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Correspondence to Ning-Ping Huang.

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Huang, NP., Yu, H., Wang, YY. et al. Efficient creation of cellular micropatterns with long-term stability and their geometric effects on cell behavior. Biointerphases 6, 143–152 (2011). https://doi.org/10.1116/1.3644381

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