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Biointerphases

Journal for Biophysical Chemistry

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Proteopolymersomes: In vitro production of a membrane protein in polymersome membranes

Biointerphases volume 6, pages153–157(2011)Cite this article

Abstract

Polymersomes are stable self-assembled architectures which mimic cell membranes. For characterization, membrane proteins can be incorporated into such bio-mimetic membranes by reconstitution methods, leading to so-called proteopolymersomes. In this work, we demonstrate the direct incorporation of a membrane protein into polymersome membranes by a cell-free expression system. Firstly, we demonstrate pore formation in the preformed polymersome membrane using α-hemolysin. Secondly, we use claudin-2, a protein involved in cell-cell interactions, to demonstrate the in vitro expression of a membrane protein into these polymersomes. Surface plasmon resonance (Biacore) binding studies with the claudin-2 proteopolymersomes and claudin-2 specific antibodies are performed to show the presence of the in vitro expressed protein in polymersome membranes.

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Affiliations

  1. Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore, 117602
    • Madhavan Nallani
    • , Mirjam Andreasson-Ochsner
    • , Cherng-Wen Darren Tan
    •  & Eva-Kathrin Sinner
  2. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Avenue, Singapore, 637551
    • Yudi Wisantoso
    •  & Susana Geifman-Shochat
  3. Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), 61 Biopolis Dr, Singapore, 138673
    • Walter Hunziker
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Nallani, M., Andreasson-Ochsner, M., Tan, C.D. et al. Proteopolymersomes: In vitro production of a membrane protein in polymersome membranes. Biointerphases 6, 153–157 (2011). https://doi.org/10.1116/1.3644384

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