- Open access
- Published:
Fibroblast adhesion on unidirectional polymeric nanofilms
Biointerphases volume 6, pages 158–163 (2011)
Abstract
Nanotextured polymeric surfaces with inclined rods reveal highly anisotropic properties concerning wetting and adhesion. In this work, we report on the interaction of fibroblast cells with these highly anisotropic materials. The authors quantified removal of adherent cells from such surfaces by a laminar flow. The critical shear force needed for cell removal from the surface depends on the inclination direction. Based on electron microscopy cross sections we deduce that interactions of cellular filopodia extending into the nanotextured surface are causing the direction depending removal.
References
P. C. Brooks, R. A. F. Clark, and D. A. Cheresh, Science 264, 569 (1994).
C. S. Chen, M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber, Science 276, 1425 (1997).
P. Martin, Science 276, 75 (1997).
M. Dembo and Y. L. Wang, Biophys. J. 76, 2307 (1999).
D. H. Kim, P. K. Wong, J. Park, A. Levchenko and Y. Sun, Ann. Rev. Biomed. Eng. 11, 203 (2009).
E. W. K. Young and C. A. Simmons, Lab Chip 10, 143 (2009).
H. Lu, L. Y. Koo, W. C. M. Wang, D. A. Lauffenburger, L. G. Griffith, and K. F. Jensen, Anal. Chem. 76, 5257 (2004).
N. Korin, A. Bransky, U. Dinnar and S. Levenberg, Lab Chip 7, 611 (2007).
B. Lincoln, F. Wottawah, S. Schinkinger, S. Ebert and J. Guck, Cell Mech. 83, 397 (2007).
N. Walter, A. Micoulet, T. Seufferlein and J. P. Spatz, Biointerphases 6, 117 (2011).
A. Manbachi, S. Shrivastava, M. Cioffi, B. G. Chung, M. Moretti, U. Demirci, M. Yliperttula and A. Khademhosseini, Lab Chip 8, 747 (2008).
S. Moon, S. K. Hasan, Y. S. Song, F. Xu, H. O. Keles, F. Manzur, S. Mikkilineni, J. W. Hong, J. Nagatomi, E. Haeggstrom, A. Khademhosseini and U. Demirci, Tissue Eng. Part C 16, 157 (2010).
L. Bocquet and J. L. Barrat, Soft Matter 3, 685 (2007).
S. R. Khetani and S. N. Bhatia, Nat. Biotechnol. 26, 120 (2008).
G. W. Schmid-Schonbein, Physiol. Rev. 70, 987 (1990).
C. Christophis, M. Grunze and A. Rosenhahn, Phys. Chem. Chem. Phys. 12, 4498 (2010).
C. Christophis, I. Taubert, G. R. Meseck, M. Schubert, M. Grunze, A. D. Ho, and A. Rosenhahn, Biophys. J. 101, 585 (2011).
M. Goldberg, R. Langer, and X. Jia, J. Biomater. Sci. 18, 241 (2007).
D. Khang, J. Lu, C. Yao, K. M. Haberstroh, and T. J. Webster, Biomaterials 29, 970 (2008).
A. J. Dulgar-Tulloch, R. Bizios, and R. W. Siegel, J. Biomed. Mater. Res. Part A 90, 586 (2009).
Y. W. Chun, D. Khang, K. M. Haberstroh, and T. J. Webster, Nanotechnology 20, (2009).
M. Arnold, E. A. Cavalcanti-Adam, R. Glass, J. Blummel, W. Eck, M. Kantlehner, H. Kessler, and J. P. Spatz, Chemphyschem 5, 383 (2004).
E. A. Cavalcanti-Adam, M. Bezler, P. Tomakidi, and J. P. Spatz, J. Bone Miner. Res. 19, S64 (2004).
E. A. Cavalcanti-Adam, A. Micoulet, J. Blummel, J. Auernheimer, H. Kessler, and J. P. Spatz, Eur. J. Cell Biol. 85, 219 (2006).
M. Cetinkaya, S. Boduroglu, and M. C. Demirel, Polymer 48, 4130 (2007).
M. C. Demirel, S. Boduroglu, M. Cetinkaya, and A. Lakhtakia, Langmuir 23, 5861 (2007).
M. C. Demirel, Colloids and Surf. 321, 121 (2008).
N. A. Malvadkar, M. J. Hancock, K. Sekeroglu, W. J. Dressick, and M. C. Demirel, Nat. Mater. 9, 1023 (2010).
S. Boduroglu, M. Cetinkaya, W. J. Dressick, A. Singh, and M. C. Demirel, Langmuir 23, 11391 (2007).
E. So, M. C. Demirel, and K. J. Wahl, J. Phys. D 43, 045403 (2010).
M. C. Demirel, M. Cetinkaya, A. Singh, and W. J. Dressick, Adv. Mater. 19, 4495 (2007).
M. Cetinkaya, N. Malvadkar, and M. C. Demirel, J. Polym. Sci., Part B: Polym. Phys. 46, 640 (2008).
M. Cetinkaya and M. C. Demirel, Chem. Vap. Deposition 15, 101 (2009).
G. Demirel, N. Malvadkar, and M. C. Demirel, Thin Solid Films 518, 4252 (2010).
J. I. Garrels and B. R. Franza, J. Biol. Chem. 264, 5283 (1989).
W. M. Deen, Analysis of Transport Phenomena (Oxford University Press, New York, 1998).
E. W. K. Young, A. R. Wheeler, and C. A. Simmons, Lab Chip 7, 1759 (2007).
A. J. Garcia, P. Ducheyne, and D. Boettiger, Biomaterials 18, 1091 (1997).
B. P. Chan, A. Chilkoti, W. M. Reichert, and G. A. Truskey, Biomaterials 24, 559 (2003).
J. M. Nieuwenhuizen and H. B. Haanstra, Philips Tech. Rev. 27, 87 (1966).
K. Christ, K. Williamson, K. Masters, and K. Turner, Biomed. Microdevices 12, 443 (2010).
D. A. Hammer and D. A. Lauffenburger, Biophys. J. 52, 475 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Christophis, C., Sekeroglu, K., Demirel, G. et al. Fibroblast adhesion on unidirectional polymeric nanofilms. Biointerphases 6, 158–163 (2011). https://doi.org/10.1116/1.3646093
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1116/1.3646093