Figure 6
From: Fluorescence modulation sensing of positively and negatively charged proteins on lipid bilayers
Protein sensing in 10 mM PBS: Line scans for microfluidic channels before and after the introduction of (left) streptavidin and (right) avidin. (a) A supported bilayer composed of 0.5 mol% ortho-rhodamine B and 5 mol% biotin-PE in POPC before and after the addition of streptavidin (blue and red, respectively). (b) A control bilayer composed of 0.5 mol% ortho-rhodamine B in POPC before and after addition of streptavidin. (c) A bilayer composed of 0.5 mol% ortho-rhodamine B and 5 mol% biotin-PE in POPC before and after the addition of avidin, and (d) a control bilayer composed of 0.5 mol% ortho-rhodamine B in POPC before and after the addition of avidin.