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Journal for Biophysical Chemistry

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Figure 3 | Biointerphases

Figure 3

From: Characterization of sample preparation methods of NIH/3T3 fibroblasts for ToF-SIMS analysis

Figure 3

Spectra from the surface of cells that were analyzed (A) frozen-hydrated, (B) freeze-dried, (C) formaldehyde-fixed followed by a light rinse and (D) formaldehyde-fixed followed by a heavy rinse. Each spectrum was normalized to the total ion intensity, and all spectra were plotted with the same y-scale. All spectra were acquired with a 250 μm × 250 μm analysis area and to a primary ion dose density of 1.0 × 1012 ions/cm2. Peaks assignments are given in the text.

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